肿瘤坏死因子-α和核因子-κB 基因变异与脓毒症。
Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis.
机构信息
Department of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, Turkey.
Clinic of Anesthesia and Reanimation, Siyami Ersek Thoracic Cardiovascular Surgery Training and Research Hospital, İstanbul, Turkey.
出版信息
Balkan Med J. 2018 Jan 20;35(1):30-35. doi: 10.4274/balkanmedj.2017.0246. Epub 2017 Aug 25.
BACKGROUND
The humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. Nuclear factor-kappa B dimers are encoded by nuclear factor-kappa B genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis factor-alpha.
AIMS
To investigate the possible effect of polymorphisms on tumour necrosis factor-alpha serum levels with clinical and prognostic parameters of sepsis by determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A) gene polymorphisms and tumour necrosis factor-alpha serum levels.
STUDY DESIGN
Case-control study.
METHODS
Seventy-two patients with sepsis and 104 healthy controls were included in the study. In order to determine the polymorphisms of nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A), polymerase chain reaction-restriction fragment length polymorphism analysis was performed and serum tumour necrosis factor-alpha levels were determined using an enzyme-linked immunosorbent assay.
RESULTS
We observed no significant differences in tumour necrosis factor-alpha serum levels between the study groups. In the patient group, an increase in the tumour necrosis factor-alpha serum levels in patients carrying the tumour necrosis factor-alpha (-308 G/A) A allele compared to those without the A allele was found to be statistically significant. Additionally, an increase in the tumour necrosis factor-alpha serum levels in patients carrying tumour necrosis factor-alpha (-308 G/A) AA genotype compared with patients carrying the AG or GG genotypes was statistically significant. No significant differences were found in these 2 polymorphisms between the patient and control groups (p>0.05).
CONCLUSION
Our results showed the AA genotype and the A allele of the tumour necrosis factor-alpha (-308 G/A) polymorphism may be used as a predictor of elevated tumour necrosis factor-alpha levels in patients with sepsis.
背景
由于组织感染和创伤性损伤，体液系统被激活，各种细胞因子被释放。核因子-κB 二聚体由核因子-κB 基因编码，调节肿瘤坏死因子-α等炎症关键蛋白的转录。
目的
通过测定核因子-κB-1-94 插入/缺失 ATTG 和肿瘤坏死因子-α（-308G/A）基因多态性及肿瘤坏死因子-α血清水平，探讨肿瘤坏死因子-α 血清水平与脓毒症临床及预后参数之间的可能关系。
研究设计
病例对照研究。
方法
本研究纳入了 72 例脓毒症患者和 104 名健康对照者。为了确定核因子-κB-1-94 插入/缺失 ATTG 和肿瘤坏死因子-α（-308G/A）的多态性，采用聚合酶链反应-限制性片段长度多态性分析，并用酶联免疫吸附试验测定血清肿瘤坏死因子-α水平。
结果
我们未观察到研究组间肿瘤坏死因子-α血清水平存在显著差异。在患者组中，与不携带 A 等位基因的患者相比，携带肿瘤坏死因子-α（-308G/A）A 等位基因的患者其肿瘤坏死因子-α 血清水平升高，差异有统计学意义。此外，与携带 AG 或 GG 基因型的患者相比，携带肿瘤坏死因子-α（-308G/A）AA 基因型的患者其肿瘤坏死因子-α 血清水平升高，差异也有统计学意义。但这两种多态性在患者组和对照组之间均无显著差异（p＞0.05）。
结论
我们的研究结果表明，肿瘤坏死因子-α（-308G/A）多态性的 AA 基因型和 A 等位基因可能可作为预测脓毒症患者肿瘤坏死因子-α水平升高的指标。
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