Biomedical Engineering Department, Faculty of New Sciences and Technologies, The University of Tehran , Tehran 14395-1561, Iran.
Biomedical Engineering Department, The University of Texas at Austin , Austin, Texas 78712, United States.
ACS Appl Mater Interfaces. 2017 Sep 27;9(38):32607-32620. doi: 10.1021/acsami.7b11291. Epub 2017 Sep 18.
Strong near-infrared (NIR) absorption of reduced graphene oxide (rGO) make this material a candidate for photothermal therapy. The use of rGO has been limited by low stability in aqueous media due to the lack of surface hydrophilic groups. We report synthesis of a novel form of reduced graphene-arginine (rGO-Arg) as a nanoprobe. Introduction of Arg to the surface of rGO not only increases the stability in aqueous solutions but also increases cancer cell uptake. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) images are recorded to characterize the morphology of rGO-Arg. Fourier transform infrared (FTIR), X-ray photoelectron spectra (XPS), Raman, and UV-vis spectroscopy are utilized to analyze the physiochemical properties of rGO-Arg. Interaction of rGO-Arg with 808 nm laser light has been evaluated by measuring the absorption cross section in response to periodically modulated intensity to minimize artifacts arising from lateral thermal diffusion with a material scattering matched to a low scattering optical standard. Cell toxicity and cellular uptake by MD-MB-231 cell lines provide supporting data for the potential application of rGO-Arg for photothermal therapy. Absorption cross-section results suggest rGO-Arg is an excellent NIR absorber that is 3.2 times stronger in comparison to GO.
还原氧化石墨烯（rGO）具有较强的近红外（NIR）吸收能力，使其成为光热疗法的候选材料。由于缺乏表面亲水性基团，rGO 在水介质中的稳定性较低，因此其应用受到限制。我们报告了一种新型还原氧化石墨烯-精氨酸（rGO-Arg）纳米探针的合成方法。在 rGO 表面引入 Arg 不仅增加了其在水溶液中的稳定性，还增加了癌细胞的摄取量。原子力显微镜（AFM）和透射电子显微镜（TEM）图像被记录下来以表征 rGO-Arg 的形态。傅里叶变换红外（FTIR）、X 射线光电子能谱（XPS）、拉曼和紫外可见光谱用于分析 rGO-Arg 的物理化学性质。通过测量响应周期性调制强度的吸收截面来评估 rGO-Arg 与 808nm 激光的相互作用，以最小化由于与低散射光学标准匹配的材料散射引起的横向热扩散产生的伪影。MD-MB-231 细胞系的细胞毒性和细胞摄取为 rGO-Arg 用于光热治疗的潜在应用提供了支持数据。吸收截面结果表明，rGO-Arg 是一种出色的近红外吸收剂，其吸收能力比 GO 强 3.2 倍。
