Zare Mehrabadi Farzaneh, Haddad Mohammad Ali, Hosseini Motlagh Najmeh Sadat, Zarei Mahmoudabadi Mohammad, Sardari Zarchi Mohsen, Haghiralsadat Bibi Fatemeh
Department of Physics, Yazd University, Yazd, Islamic Republic of Iran.
Laser Spectroscopy Research Laboratory (LSRL), Department of Physics, Yazd University, Yazd, Islamic Republic of Iran.
Lasers Med Sci. 2025 Jan 28;40(1):50. doi: 10.1007/s10103-025-04311-3.
Photothermal therapy, in which a laser is an effective tool, is a promising method for cancer treatment. Laser parameters, including power, irradiation time, type of laser radiation (continuous or chopped), and the concentration of the photothermal agent, can affect the efficiency of this method. Therefore, this study investigated and compared the effects of different laser parameters on the efficiency of photothermal treatment for cervical cancer, which is the fourth most prevalent cancer in women. In addition, we investigated the properties of graphene oxide (GO) synthesized as a photothermal agent under laser radiation, and its effectiveness in achieving the desired therapeutic temperature. This study examined and compared the effects of temperature, nanoparticle concentration, irradiation time, and laser power to understand their impact on heat transfer. The toxicity of graphene oxide at different concentrations in HeLa cancer cells was also evaluated. These results demonstrated low toxicity, particularly after 24 h, with approximately 10% toxicity. The study explored mortality under laser irradiation at various powers and time intervals using continuous and chopped beam irradiation. In addition, a model for temperature prediction using a regression tree was presented. Finally, the combined photothermal effects of graphene oxide and laser irradiation were investigated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test results reveal significant effects, with a mortality rate of 90% in continuous radiation with a concentration of 0.3 mg/ml and 75% in chopped beam irradiation with concentrations of 0.3 and 0.4 mg/ml. A regression tree model was developed to predict temperature changes based on the GO concentration, laser power, and irradiation time, providing valuable insights for optimizing photothermal therapy parameters. Statistical analysis showed that the combined effect of graphene oxide with continuous laser irradiation was more effective than chopped-beam laser irradiation. However, the chopped-beam irradiation method is expected to cause less damage to surrounding tissues. These findings indicate that photothermal therapy with graphene oxide, chopped, and continuous laser irradiation can potently treat HeLa cancer cells and pave the way for further exploration of targeted cancer treatments.
光热疗法是一种很有前景的癌症治疗方法，其中激光是一种有效的工具。激光参数，包括功率、照射时间、激光辐射类型（连续或斩波）以及光热剂的浓度，都会影响该方法的效率。因此，本研究调查并比较了不同激光参数对子宫颈癌光热治疗效率的影响，子宫颈癌是女性中第四大常见癌症。此外，我们研究了在激光辐射下合成的作为光热剂的氧化石墨烯（GO）的特性，以及其在达到所需治疗温度方面的有效性。本研究检测并比较了温度、纳米颗粒浓度、照射时间和激光功率的影响，以了解它们对热传递的影响。还评估了不同浓度的氧化石墨烯在HeLa癌细胞中的毒性。这些结果表明毒性较低，特别是在24小时后，毒性约为10%。该研究探讨了在不同功率和时间间隔下使用连续和斩波光束照射时激光照射下的死亡率。此外，还提出了一种使用回归树进行温度预测的模型。最后，研究了氧化石墨烯与激光照射的联合光热效应。MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）试验结果显示出显著效果，在浓度为0.3mg/ml的连续辐射中死亡率为90%，在浓度为0.3mg/ml和0.4mg/ml的斩波光束照射中死亡率为75%。开发了一种回归树模型，以根据GO浓度、激光功率和照射时间预测温度变化，为优化光热治疗参数提供了有价值的见解。统计分析表明，氧化石墨烯与连续激光照射的联合效果比斩波光束激光照射更有效。然而，斩波光束照射方法预计对周围组织造成的损伤较小。这些发现表明，氧化石墨烯与斩波和连续激光照射的光热疗法可以有效治疗HeLa癌细胞，并为进一步探索靶向癌症治疗铺平道路。
