关于理解体内中介体功能机制：聚焦于起始前复合物组装。
Toward understanding of the mechanisms of Mediator function in vivo: Focus on the preinitiation complex assembly.
作者信息
Eychenne Thomas, Werner Michel, Soutourina Julie
机构信息
a Institute for Integrative Biology of the Cell (I2BC), Institute of Life Sciences Frédéric Joliot, CEA, CNRS , Univ. Paris Sud, University Paris Saclay , Gif-sur-Yvette , France.
b Institut Pasteur, (Epi)genomics of Animal Development Unit , Development and Stem Cell Biology Department, CNRS UMR3778 , Paris , France.
出版信息
Transcription. 2017;8(5):328-342. doi: 10.1080/21541264.2017.1329000. Epub 2017 Aug 25.
Abstract
Mediator is a multisubunit complex conserved in eukaryotes that plays an essential coregulator role in RNA polymerase (Pol) II transcription. Despite intensive studies of the Mediator complex, the molecular mechanisms of its function in vivo remain to be fully defined. In this review, we will discuss the different aspects of Mediator function starting with its interactions with specific transcription factors, its recruitment to chromatin and how, as a coregulator, it contributes to the assembly of transcription machinery components within the preinitiation complex (PIC) in vivo and beyond the PIC formation.
摘要
中介体是真核生物中保守的多亚基复合物，在RNA聚合酶（Pol）II转录中起重要的共调节因子作用。尽管对中介体复合物进行了深入研究，但其在体内的功能分子机制仍有待充分阐明。在本综述中，我们将讨论中介体功能的不同方面，首先是其与特定转录因子的相互作用、其被招募到染色质上的过程，以及作为共调节因子，它如何在体内起始前复合物（PIC）内及PIC形成之外促进转录机制组件的组装。
相似文献
1
Toward understanding of the mechanisms of Mediator function in vivo: Focus on the preinitiation complex assembly.
Transcription. 2017;8(5):328-342. doi: 10.1080/21541264.2017.1329000. Epub 2017 Aug 25.
2
Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo.
Nucleic Acids Res. 2015 Oct 30;43(19):9214-31. doi: 10.1093/nar/gkv782. Epub 2015 Aug 3.
3
Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.
Genes Dev. 2016 Sep 15;30(18):2119-2132. doi: 10.1101/gad.285775.116. Epub 2016 Sep 29.
4
Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.
Mol Cell. 2021 Sep 2;81(17):3560-3575.e6. doi: 10.1016/j.molcel.2021.07.022. Epub 2021 Aug 9.
5
Evidence that Mediator is essential for Pol II transcription, but is not a required component of the preinitiation complex in vivo.
Elife. 2017 Jul 12;6:e28447. doi: 10.7554/eLife.28447.
6
Control of gene transcription by Mediator in chromatin.
Semin Cell Dev Biol. 2011 Sep;22(7):735-40. doi: 10.1016/j.semcdb.2011.08.004. Epub 2011 Aug 12.
7
Mediator and SAGA have distinct roles in Pol II preinitiation complex assembly and function.
Cell Rep. 2012 Nov 29;2(5):1061-7. doi: 10.1016/j.celrep.2012.10.019. Epub 2012 Nov 21.
8
The RNA polymerase II transcriptional machinery and its epigenetic context.
Subcell Biochem. 2013;61:237-59. doi: 10.1007/978-94-007-4525-4_11.
9
Structure of the human Mediator-bound transcription preinitiation complex.
Science. 2021 Apr 2;372(6537):52-56. doi: 10.1126/science.abg3074. Epub 2021 Mar 11.
10
Mediator, known as a coactivator, can act in transcription initiation in an activator-independent manner in vivo.
Biochim Biophys Acta Gene Regul Mech. 2018 Aug;1861(8):687-696. doi: 10.1016/j.bbagrm.2018.07.001. Epub 2018 Jul 7.
引用本文的文献
1
Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes.
Proc Natl Acad Sci U S A. 2021 Sep 28;118(39). doi: 10.1073/pnas.2108751118.
2
A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.
Mol Cell. 2021 Jan 7;81(1):183-197.e6. doi: 10.1016/j.molcel.2020.11.010. Epub 2020 Dec 4.
3
The nuclear cap-binding complex as choreographer of gene transcription and pre-mRNA processing.
Genes Dev. 2020 Sep 1;34(17-18):1113-1127. doi: 10.1101/gad.339986.120.
4
Selective Mediator dependence of cell-type-specifying transcription.
Nat Genet. 2020 Jul;52(7):719-727. doi: 10.1038/s41588-020-0635-0. Epub 2020 Jun 1.
5
Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin.
Nucleic Acids Res. 2019 Sep 26;47(17):8988-9004. doi: 10.1093/nar/gkz598.
6
The C-Terminal Transactivation Domain of STAT1 Has a Gene-Specific Role in Transactivation and Cofactor Recruitment.
Front Immunol. 2018 Dec 6;9:2879. doi: 10.3389/fimmu.2018.02879. eCollection 2018.
7
Disruption of cardiac Med1 inhibits RNA polymerase II promoter occupancy and promotes chromatin remodeling.
Am J Physiol Heart Circ Physiol. 2019 Feb 1;316(2):H314-H325. doi: 10.1152/ajpheart.00580.2018. Epub 2018 Nov 21.
8
Oncogenic exon 2 mutations in Mediator subunit MED12 disrupt allosteric activation of cyclin C-CDK8/19.
J Biol Chem. 2018 Mar 30;293(13):4870-4882. doi: 10.1074/jbc.RA118.001725. Epub 2018 Feb 13.
本文引用的文献
1
Mediator structure and rearrangements required for holoenzyme formation.
Nature. 2017 Apr 13;544(7649):196-201. doi: 10.1038/nature21393. Epub 2017 Mar 1.
2
Rapid dynamics of general transcription factor TFIIB binding during preinitiation complex assembly revealed by single-molecule analysis.
Genes Dev. 2016 Sep 15;30(18):2106-2118. doi: 10.1101/gad.285395.116.
3
Mediator binding to UASs is broadly uncoupled from transcription and cooperative with TFIID recruitment to promoters.
EMBO J. 2016 Nov 15;35(22):2435-2446. doi: 10.15252/embj.201695020. Epub 2016 Oct 20.
4
Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo.
Mol Cell. 2016 Nov 3;64(3):455-466. doi: 10.1016/j.molcel.2016.09.002. Epub 2016 Oct 20.
5
Mediator Undergoes a Compositional Change during Transcriptional Activation.
Mol Cell. 2016 Nov 3;64(3):443-454. doi: 10.1016/j.molcel.2016.09.015. Epub 2016 Oct 20.
6
Opposing effects of Elk-1 multisite phosphorylation shape its response to ERK activation.
Science. 2016 Oct 14;354(6309):233-237. doi: 10.1126/science.aad1872.
7
Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.
Genes Dev. 2016 Sep 15;30(18):2119-2132. doi: 10.1101/gad.285775.116. Epub 2016 Sep 29.
8
Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
Cell. 2016 Sep 8;166(6):1411-1422.e16. doi: 10.1016/j.cell.2016.08.050.
9
A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting.
Nat Commun. 2016 Jul 27;7:12248. doi: 10.1038/ncomms12248.
10
Inhibiting fungal multidrug resistance by disrupting an activator-Mediator interaction.
Nature. 2016 Feb 25;530(7591):485-9. doi: 10.1038/nature16963. Epub 2016 Feb 17.
Zotero 插件