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水稻细胞色素f基因的核苷酸序列以及该基因附近的序列变异情况。

Nucleotide sequence of the rice cytochrome f gene and the presence of sequence variation near this gene.

作者信息

Wu N, Côté J C, Wu R

出版信息

Gene. 1986;50(1-3):271-8. doi: 10.1016/0378-1119(86)90331-8.

DOI:10.1016/0378-1119(86)90331-8
PMID:2884170
Abstract

The rice (Oryza sativa L. var. Labelle) chloroplast (cp) gene encoding cytochrome f has been isolated and sequenced. The coding region of this rice gene displays 95.1%, 85.3% and 85.2% nucleotide sequence homology with that of wheat, pea and spinach, respectively. To examine the cpDNA sequence variation in rice, cpDNA from Labelle and its parents, Belle Patna and Dawn was compared. Using the cytochrome f gene as the probe for hybridization, we found several differences in the size and number of restriction fragments in the cp genome of three rice varieties. An additional restriction fragment found in the Belle Patna cp suggests that this cp genome is either heterogeneous or contains two copies of cytochrome f gene per cpDNA.

摘要

已分离并测序了编码细胞色素f的水稻(Oryza sativa L. var. Labelle)叶绿体(cp)基因。该水稻基因的编码区与小麦、豌豆和菠菜的编码区核苷酸序列同源性分别为95.1%、85.3%和85.2%。为研究水稻叶绿体DNA(cpDNA)序列变异,比较了Labelle及其亲本Belle Patna和Dawn的cpDNA。以细胞色素f基因为杂交探针,我们发现三个水稻品种cp基因组中限制性片段的大小和数量存在若干差异。在Belle Patna cp中发现的一个额外限制性片段表明,该cp基因组要么是异质的,要么每个cpDNA含有两个细胞色素f基因拷贝。

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引用本文的文献

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Photosynth Res. 1988 Jul;17(1-2):125-44. doi: 10.1007/BF00047685.
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The cloning and sequencing of Synechococcus sp. PCC 7002 petCA operon: Implications for the cytochrome c-553 binding domain of cytochrome f.聚球藻 PCC 7002 petCA 操纵子的克隆和测序:对细胞色素 f 的细胞色素 c-553 结合域的影响。
Photosynth Res. 1991 Dec;30(2-3):71-84. doi: 10.1007/BF00042005.
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Cytochrome f: Structure, function and biosynthesis.细胞色素 f:结构、功能与生物合成。
Photosynth Res. 1992 Dec;34(3):359-74. doi: 10.1007/BF00029811.
4
Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs.核苷酸替换率在植物线粒体DNA、叶绿体DNA和核DNA之间差异极大。
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9054-8. doi: 10.1073/pnas.84.24.9054.
5
N.m.r., e.p.r. and magnetic-c.d. studies of cytochrome f. Identity of the haem axial ligands.细胞色素f的核磁共振、电子顺磁共振和磁圆二色性研究。血红素轴向配体的鉴定。
Biochem J. 1988 Dec 1;256(2):571-7. doi: 10.1042/bj2560571.
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Structure and expression of cytochrome f in an Oenothera plastome mutant.
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