Kao T, Moon E, Wu R
Nucleic Acids Res. 1984 Oct 11;12(19):7305-15. doi: 10.1093/nar/12.19.7305.
We have isolated and sequenced the cytochrome oxidase subunit II gene from rice (Oryza sativa L. var Labelle). The overall structural organization of this gene is very similar to that of the maize gene. This gene contains an intron in a position identical to the intron in the maize gene. However, the intron in the rice gene is longer than that of the maize gene largely due to a 461 bp insertion sequence, which has inverted repeats at its termini and is flanked by direct repeats, characteristic of transposable elements. Apart from this insertion sequence, the remainder of the intron sequence is strikingly homologous to that of maize (98.6% homology), suggesting a possible functional or structural role. The coding regions of the two genes exhibit 99.5% nucleotide sequence homology and their deduced amino acid sequences are identical. Similarly, the 3'-noncoding regions, except for several small insertions and deletions, show complete sequence homology. On the contrary, no sequence homology is detected in the 5'-noncoding regions.
我们已经从水稻（Oryza sativa L. var Labelle）中分离并测序了细胞色素氧化酶亚基II基因。该基因的整体结构组织与玉米基因非常相似。此基因在与玉米基因内含子相同的位置含有一个内含子。然而，水稻基因中的内含子比玉米基因中的内含子长，这主要是由于一个461 bp的插入序列，该序列在其末端具有反向重复序列，并由正向重复序列侧翼，这是转座元件的特征。除了这个插入序列外，内含子序列的其余部分与玉米的内含子序列具有惊人的同源性（98.6%的同源性），表明其可能具有功能或结构作用。这两个基因的编码区表现出99.5%的核苷酸序列同源性，并且它们推导的氨基酸序列是相同的。同样，3'-非编码区除了几个小的插入和缺失外，显示出完全的序列同源性。相反，在5'-非编码区未检测到序列同源性。
