一种基于醛基封端离子液体的新型无标记电化学免疫传感器。
A novel label-free electrochemical immunosensor based on aldehyde-terminated ionic liquid.
作者信息
Shen Youming, Shen Guangyu, Zhang Youyu, Zhang Chunxiang, Li Haitao
机构信息
Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Material Engineering, Hunan University of Arts and Science, Changde 415000, PR China; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Material Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
出版信息
Talanta. 2017 Dec 1;175:347-351. doi: 10.1016/j.talanta.2017.07.023. Epub 2017 Jul 11.
In this paper, aldehyde-terminated ionic liquid was synthesized and used to fabricate a novel label-free electrochemical immunosensor for the determination of hepatitis B surface antigen. This sensor was formed from a gold electrode which was modified with a composite film containing the aldehyde-functionalized ionic liquid incorporated into nafion and anti-hepatitis B surface antigen antibody probes. Aldehyde-functionalized ionic liquid was used not only as a substrate for antibody immobilization, but also as a good material with high electrical conductivity, which greatly simplified the process of sensor fabrication. The proposed sensor exhibited a good performance in a linear range from 0.05 to 15ngmL with a detection limit of 20pgmL (S/N=3) and provided a novel strategy for clinical detection due to satisfying stability, selectivity and reproducibility.
本文合成了醛基封端的离子液体，并将其用于制备一种新型的无标记电化学免疫传感器，用于检测乙型肝炎表面抗原。该传感器由金电极构成，金电极用复合膜修饰，该复合膜包含掺入到全氟磺酸离子交换膜中的醛基功能化离子液体和抗乙型肝炎表面抗原抗体探针。醛基功能化离子液体不仅用作抗体固定的底物，还用作具有高电导率的良好材料，这大大简化了传感器的制备过程。所提出的传感器在0.05至15ng/mL的线性范围内表现出良好的性能，检测限为20pg/mL（S/N=3），并且由于具有令人满意的稳定性、选择性和重现性，为临床检测提供了一种新策略。
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