基于含二醛离子液体作为抗体固定化新型连接剂的无标记电化学免疫传感器
Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization.
作者信息
Shen Youming, Shen Guangyu, Zhang Youyu
机构信息
Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, P. R. China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China.
出版信息
ACS Omega. 2018 Sep 30;3(9):11227-11232. doi: 10.1021/acsomega.8b01298. Epub 2018 Sep 14.
Demand for label-free electrochemical immunosensor has resulted in extensive research in improving the conductivity of a sensing interface and antibody immobilization. In this paper, an electrochemical immunosensor for prostate specific antigen based on dialdehyde-functionalized ionic liquid used as a novel linking reagent to replace glutaraldehyde for the antibody immobilization is described. The novel linking reagent enhanced the conductivity of the sensing interface. Thus, the proposed immunosensor had a wider linear range of 0.05-30 ng mL, with a lower detection limit of 0.04 ng mL compared with the immunosensor based on glutaraldehyde for the antibody immobilization.
对无标记电化学免疫传感器的需求促使人们对提高传感界面的导电性和抗体固定化进行了广泛研究。本文描述了一种基于二醛功能化离子液体的前列腺特异性抗原电化学免疫传感器，该离子液体用作新型连接试剂以取代戊二醛进行抗体固定化。这种新型连接试剂提高了传感界面的导电性。因此，与基于戊二醛进行抗体固定化的免疫传感器相比，所提出的免疫传感器具有0.05 - 30 ng/mL的更宽线性范围，检测下限低至0.04 ng/mL。
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