Shen Qihui, Liu Man, Lü Yang, Zhang Dawei, Cheng Zhenyu, Liu Yan, Gao Huajing, Jin Zhaohui
Department of Chemistry and Pharmaceutical Engineering and Department of Petrochemical Technology, Jilin Institute of Chemical Technology, Jilin 132022, P. R. China.
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China.
ACS Omega. 2019 Jul 9;4(7):11888-11892. doi: 10.1021/acsomega.9b01152. eCollection 2019 Jul 31.
A label-free electrochemical immunosensor for cardiac troponin I was prepared by using a helical carbon nanotube-supported aldehyde-functionalized ionic liquid. Because of the good conductivity of ionic liquid and helical carbon nanotubes, high sensitivity of the immunosensor was obtained. Functionalized ionic liquid provided binding sites for antibody, which simplified the process of sensor construction. Cardiac troponin I was detected by this immunosensor with a linear range of 0.05-30 ng/mL and a detection limit of 0.03 ng/mL. The electrochemical immunosensor had satisfactory reproducibility, high sensitivity, and acceptable specificity.
通过使用螺旋碳纳米管负载的醛功能化离子液体制备了一种用于心肌肌钙蛋白I的无标记电化学免疫传感器。由于离子液体和螺旋碳纳米管具有良好的导电性，该免疫传感器获得了高灵敏度。功能化离子液体为抗体提供了结合位点，简化了传感器的构建过程。用该免疫传感器检测心肌肌钙蛋白I，线性范围为0.05 - 30 ng/mL，检测限为0.03 ng/mL。该电化学免疫传感器具有令人满意的重现性、高灵敏度和可接受的特异性。
