Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, People's Republic of China.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, People's Republic of China.
Fish Shellfish Immunol. 2017 Oct;69:195-199. doi: 10.1016/j.fsi.2017.08.025. Epub 2017 Aug 24.
Different haemocyte types have been reported to play diverse roles in immune defense of shrimp. To investigate the roles of the three haemocyte types [hyaline cells (HC), semigranular cells (SGC) and granular cells (GC)] of shrimp in immune responses against lipopolysaccharide (LPS), percentage, non-specific esterase activity (EA), reactive oxygen species (ROS) production and nitric oxide (NO) production of the three haemocyte subpopulations were analyzed in LPS-injected Penaeus monodon using flow cytometry. Results showed that percentage of HC increased after 3 h injection, and returned to the original level after 48 h. Proportion of SGC and GC reduced after 6-36 h and 3-12 h respectively, and recovered to the initial level after 48 and 24 h respectively. Loss of SGC and GC might be related to degranulation to release proPO system, and degranulation of GC seemed more sensitive to LPS stimulation. EA of both HC and SGC improved after 3-6 h injection, while EA of GC was induced after 3-24 h. No significant effect of LPS injection could be found in ROS production and NO production of HC. Enhanced ROS levels was observed in SGC and GC after 3-24 h and 3-36 h respectively, and NO production of SGC and GC improved after 3-48 h injection. These results demonstrated that SGC and GC possessed strong capabilities for LPS-induced EA, ROS production and NO production, while HC only displayed EA response to LPS, suggesting that GC and SGC play the main role in immune defense of shrimp against Gram-negative bacteria.
不同类型的血细胞在虾的免疫防御中发挥着不同的作用。为了研究虾的三种血细胞类型（透明细胞[HC]、半颗粒细胞[SGC]和颗粒细胞[GC]）在对脂多糖（LPS）的免疫反应中的作用，使用流式细胞术分析了 LPS 注射后凡纳滨对虾三种血细胞亚群的百分比、非特异性酯酶活性（EA）、活性氧（ROS）产生和一氧化氮（NO）产生。结果表明，HC 的百分比在注射后 3 h 增加，48 h 后恢复到原始水平。SGC 和 GC 的比例分别在 6-36 h 和 3-12 h 后降低，48 和 24 h 后分别恢复到初始水平。SGC 和 GC 的损失可能与脱颗粒释放 proPO 系统有关，而 GC 的脱颗粒对 LPS 刺激似乎更为敏感。HC 和 SGC 的 EA 在注射后 3-6 h 提高，而 GC 的 EA 在注射后 3-24 h 被诱导。LPS 注射对 HC 的 ROS 产生和 NO 产生没有明显影响。在 3-24 h 和 3-36 h 时观察到 SGC 和 GC 的 ROS 水平增强，在 3-48 h 时 SGC 和 GC 的 NO 产生提高。这些结果表明，SGC 和 GC 具有较强的 LPS 诱导 EA、ROS 产生和 NO 产生能力，而 HC 仅对 LPS 表现出 EA 反应，提示 GC 和 SGC 在虾对革兰氏阴性菌的免疫防御中起主要作用。
