Pardoll D M, Fowlkes B J, Lechler R I, Germain R N, Schwartz R H
J Exp Med. 1987 Jun 1;165(6):1624-38. doi: 10.1084/jem.165.6.1624.
In situ hybridization was used to investigate the expression of T cell receptor (TCR) alpha, beta, and gamma mRNAs in developing fetal and adult precursor thymocytes. gamma transcription was observed at the earliest time tested (day 12), followed by beta 12 h later, and TCR alpha on day 16. The early beta transcripts appeared to be from unrearranged or incompletely rearranged (D-J-C) beta loci. V beta region transcription was first detectable on day 14 and transcription of different V beta genes was induced at different times. These results delineate a schedule sequence of TCR gene activation, which begins within 1 d after entry of stem cells into the fetal thymus.
采用原位杂交技术研究了胎儿和成年前体胸腺细胞发育过程中T细胞受体（TCR）α、β和γ mRNA的表达情况。在最早检测的时间点（第12天）观察到γ转录，12小时后出现β转录，而TCR α在第16天出现。早期的β转录本似乎来自未重排或不完全重排的（D-J-C）β基因座。Vβ区域转录最早在第14天可检测到，不同Vβ基因的转录在不同时间被诱导。这些结果描绘了TCR基因激活的时间表序列，该序列在干细胞进入胎儿胸腺后1天内开始。
