Marbouty Martial, Koszul Romain
Groupe Régulation Spatiale des Génomes, Département Génomes et Génétique, Institut Pasteur, 28 rue du Docteur Roux, 75724, Paris Cedex 15, France.
CNRS, UMR 3525, 75724, Paris Cedex 15, France.
Methods Mol Biol. 2017;1624:75-84. doi: 10.1007/978-1-4939-7098-8_7.
This methods article described a protocol aiming at generating chromosome contact maps of bacterial species using a genome-wide derivative of the chromosome conformation capture (3C) technique. The approach is readily applicable on a broad variety of gram + and gram-bacterial species. It describes and addresses known caveats and technicalities associated with the technique, and should be of interest to any laboratory interested to perform a multiscale analysis of the genome structure of its species of interest.
这篇方法学文章描述了一种方案，旨在使用染色体构象捕获（3C）技术的全基因组衍生方法生成细菌物种的染色体接触图谱。该方法很容易应用于多种革兰氏阳性菌和革兰氏阴性菌物种。它描述并解决了与该技术相关的已知注意事项和技术细节，对于任何有兴趣对其感兴趣物种的基因组结构进行多尺度分析的实验室来说都应该是有价值的。
