Institut Pasteur, Unité Régulation Spatiale des Génomes, CNRS UMR 3525, 5015 Paris, France.
STAR Protoc. 2021 May 5;2(2):100512. doi: 10.1016/j.xpro.2021.100512. eCollection 2021 Jun 18.
Chromosome conformation capture (Hi-C) has become a routine method for probing the 3D organization of genomes. However, when applied to bacteria and archaea, current protocols are expensive and limited in their resolution. By dissecting the different steps of published eukaryotic and prokaryotic Hi-C protocols, we have developed a cost- and time-effective approach to generate high-resolution (down to 500 bp - 1 kb) contact matrices of both bacteria and archaea genomes. For complete details on the use and execution of this protocol, please refer to Cockram et al. (2020).
染色体构象捕获（Hi-C）已成为研究基因组三维结构的常规方法。然而，当应用于细菌和古菌时，当前的方案昂贵且分辨率有限。通过剖析已发表的真核生物和原核生物 Hi-C 方案的不同步骤，我们开发了一种经济高效的方法，可生成细菌和古菌基因组的高分辨率（低至 500 bp-1 kb）接触矩阵。有关此方案使用和执行的完整详细信息，请参见 Cockram 等人。（2020）。
