Ly C Hai, Ryall James G
Stem Cell Metabolism and Regenerative Medicine Group, Basic and Clinical Myology Laboratory, The University of Melbourne, Parkville, VIC, 3010, Australia.
Stem Cell Metabolism and Regenerative Medicine Group, Basic and Clinical Myology Laboratory, Department of Physiology, School of Biomedical Sciences, The University of Melbourne, Melbourne, VIC, 3010, Australia.
Methods Mol Biol. 2017;1668:61-73. doi: 10.1007/978-1-4939-7283-8_5.
Skeletal muscle stem cells (MuSCs) derived from the somatic mesoderm play a critical role in successful muscle regeneration following injury and trauma. MuSCs have been found to undergo rapid changes in metabolism following a change in cell state, such as that which occurs during the transition from quiescence to an actively proliferating state. There is mounting evidence that metabolism is critically important in the regulation of quiescence, activation, and differentiation and thus the development of new techniques that aim to further probe the metabolism of MuSCs is essential. The Seahorse XF Bioanalyzer is a powerful tool that simultaneously measures the extracellular rate of change in oxygen partial pressure and pH, providing a method to measure mitochondrial respiration and lactate production. In this chapter, we describe the use of key metabolic inhibitors that allow for the investigation of mitochondrial substrate utilization in primary MuSCs.
源自体壁中胚层的骨骼肌干细胞（MuSCs）在损伤和创伤后的成功肌肉再生中起关键作用。已发现MuSCs在细胞状态发生变化后，如从静止状态转变为活跃增殖状态时，其代谢会发生快速变化。越来越多的证据表明，代谢在静止、激活和分化的调节中至关重要，因此开发旨在进一步探究MuSCs代谢的新技术至关重要。海马XF生物分析仪是一种强大的工具，可同时测量氧分压和pH值的细胞外变化率，提供一种测量线粒体呼吸和乳酸产生的方法。在本章中，我们描述了使用关键代谢抑制剂来研究原代MuSCs中线粒体底物利用的方法。
