Asano Toshifumi, Ishizuka Toru, Yawo Hiromu
Department of Cell Biology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan.
Methods Mol Biol. 2017;1668:135-145. doi: 10.1007/978-1-4939-7283-8_10.
Optogenetic techniques are powerful tools for manipulating biological processes in identified cells using light under high temporal and spatial resolutions. Here, we describe an optogenetic training strategy to promote morphological maturation and functional development of skeletal muscle cells in vitro. Optical stimulation with a rhythmical frequency facilitates specific structural alignment of sarcomeric proteins. Optical stimulation also depolarizes the membrane potential, and induces contractile responses in synchrony with the given pattern of light pulses. These results suggest that optogenetic techniques can be employed to manipulate activity-dependent processes during myogenic development and control contraction of photosensitive skeletal muscle cells with high temporal and special precision.
光遗传学技术是用于在高时间和空间分辨率下利用光操纵特定细胞中生物过程的强大工具。在此，我们描述了一种光遗传学训练策略，以促进体外骨骼肌细胞的形态成熟和功能发育。以节律性频率进行光刺激有助于肌节蛋白的特定结构排列。光刺激还会使膜电位去极化，并与给定的光脉冲模式同步诱导收缩反应。这些结果表明，光遗传学技术可用于在成肌发育过程中操纵依赖于活动的过程，并以高时间和空间精度控制光敏骨骼肌细胞的收缩。
