Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Japan.
Biotechnol Bioeng. 2012 Jan;109(1):199-204. doi: 10.1002/bit.23285. Epub 2011 Aug 18.
As the skeletal muscle cell is an efficient force transducer, it has been incorporated in bio-microdevices using electrical field stimulation for generating contractile patterns. To improve both the spatial and temporal resolutions, we made photosensitive skeletal muscle cells from murine C2C12 myoblasts, which express channelrhodopsin-2 (ChR2), one of archaea-type rhodopsins derived from green algae Chlamydomonas reinhardtii. The cloned ChR2-expressing C2C12 myoblasts were made and fused with untransfected C2C12 to form multinucleated myotubes. The maturation of myotubes was facilitated by electrical field stimulation. Blue LED light pulse depolarized the membrane potential of a ChR2-expressing myotube and eventually evoked an action potential. It also induced a twitch-like contraction in a concurrent manner. A contraction pattern was thus made with a given pattern of LED pulses. This technique would have many applications in the bioengineering field, such as wireless drive of muscle-powered actuators/microdevices.
由于骨骼肌细胞是一种高效的力传感器，因此它已被整合到生物微器件中，使用电场刺激来产生收缩模式。为了提高空间和时间分辨率，我们使用表达通道视紫红质-2（ChR2）的鼠源 C2C12 成肌细胞制造了光敏骨骼肌细胞，ChR2 是一种源自绿藻莱茵衣藻的古细菌型视紫红质。克隆的表达 ChR2 的 C2C12 成肌细胞被制造出来，并与未转染的 C2C12 融合形成多核肌管。肌管的成熟通过电场刺激来促进。蓝色 LED 光脉冲使表达 ChR2 的肌管的膜电位去极化，并最终引发动作电位。它还以协同的方式引起抽搐样收缩。因此，通过给定的 LED 脉冲模式产生了收缩模式。这项技术将在生物工程领域有许多应用，例如肌肉驱动的执行器/微器件的无线驱动。
