Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
Curr Opin Virol. 2017 Oct;26:132-140. doi: 10.1016/j.coviro.2017.07.031. Epub 2017 Aug 23.
Plant RNA viruses are widespread pathogens that need to interact intricately with their hosts to co-opt numerous cellular factors to facilitate their replication. Currently, there are only a limited number of plant resistance genes against a limited number of viruses. To develop novel antiviral approaches, the interaction network between the given virus and the host cell could be targeted. Yeast (Saccharomyces cerevisiae) has been developed as a surrogate host for tomato bushy stunt virus (TBSV), allowing systematic genome-wide screens to identify both susceptibility and restriction factors for TBSV. Importantly, pro-viral or antiviral functions of several of the characterized yeast proteins have been validated in plant hosts. This paper describes how yeast susceptibility and restriction factors of TBSV could be used as antiviral approaches. The gained knowledge on host factors could lead to novel, inducible, broad-range, and durable antiviral tools against plant viruses.
植物 RNA 病毒是广泛存在的病原体，它们需要与宿主细胞错综复杂地相互作用，以劫持众多细胞因子来促进其复制。目前，针对有限数量的病毒，仅有有限数量的植物抗性基因。为了开发新的抗病毒方法，可以针对特定病毒与宿主细胞之间的相互作用网络进行靶向治疗。酵母（酿酒酵母）已被开发为番茄丛矮病毒（TBSV）的替代宿主，允许进行系统的全基因组筛选，以鉴定 TBSV 的易感性和限制因子。重要的是，几种已鉴定的酵母蛋白的促病毒或抗病毒功能已在植物宿主中得到验证。本文介绍了如何将 TBSV 的酵母易感性和限制因子用作抗病毒方法。关于宿主因子的知识可以为植物病毒提供新型、诱导型、广谱和持久的抗病毒工具。
