de Lima T B, Paz A H R, Rados P V, Leonardi R, Bufo P, Pedicillo M C, Santoro A, Cagiano S, Aquino G, Botti G, Pannone G, Visioli F
Department of Oral Pathology - Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brazil.
Experimental Research Center, Hospital de Clínicas de Porto Alegre, Rio Grande do Sul, Brazil.
Pathol Res Pract. 2017 Sep;213(9):1072-1077. doi: 10.1016/j.prp.2017.07.027. Epub 2017 Aug 5.
The aim of this study was to evaluate the levels of autophagy in oral leukoplakia and squamous cell carcinoma and to correlate with clinical pathological features, as well as, the evolution of these lesions.
7 Normal oral mucosa, 51 oral leukoplakias, and 120 oral squamous cell carcinomas (OSCC) were included in the study. Histological sections of the mucosa and leukoplakias were evaluated throughout their length, while the carcinomas were evaluated using Tissue Microarray. After the immunohistochemical technique, LC3-II positive cells were quantified in the different epithelial layers of the mucosa and leukoplakias and in the microarrays of the squamous cell carcinomas. The correlation between positive cells with the different clinical-pathological variables and with the evolution of the lesions was tested using the t test, ANOVA, and Kaplan-Meier survival analysis.
We observed increased levels of autophagy in the oral squamous cell carcinomas (p<0.001) in relation to the other groups, but without any association with poorer evolution or survival of these patients. Among the leukoplakias, we observed a higher percentage of positive cells in the intermediate layer of the dysplastic leukoplakias (p=0.0319) and in the basal layer of lesions with poorer evolution (p=0.0133).
The levels of autophagy increased during the process of oral carcinogenesis and are correlated with poorer behavior of the leukoplakias.
本研究旨在评估口腔白斑和鳞状细胞癌中的自噬水平，并将其与临床病理特征以及这些病变的演变相关联。
本研究纳入了7例正常口腔黏膜、51例口腔白斑和120例口腔鳞状细胞癌（OSCC）。对黏膜和白斑的组织学切片进行全长评估，而对癌组织则使用组织芯片进行评估。采用免疫组织化学技术后，对黏膜和白斑的不同上皮层以及鳞状细胞癌的芯片中LC3-II阳性细胞进行定量。使用t检验、方差分析和Kaplan-Meier生存分析来检验阳性细胞与不同临床病理变量以及病变演变之间的相关性。
与其他组相比，我们观察到口腔鳞状细胞癌中的自噬水平升高（p<0.001），但与这些患者较差的病情演变或生存率无任何关联。在白斑中，我们观察到发育异常的白斑中间层（p=0.0319）和病情演变较差的病变基底层（p=0.0133）中阳性细胞的百分比更高。
自噬水平在口腔癌发生过程中升高，并且与白斑的较差行为相关。
