Zhou Lu, Zhang Yajie, Ma Yong
Department of Chemistry, School of Forensic Medicine, China Medical University Shenyang 110122 China
Department of Gastroenterology, Shengjing Hospital of China Medical University Shenyang 110004 China.
Nanoscale Adv. 2022 Aug 19;4(19):4059-4065. doi: 10.1039/d2na00446a. eCollection 2022 Sep 27.
In recent years, diverse redox-responsive drug delivery systems have emerged to prevent premature drug release and reduce drug toxicity in the human body in cancer treatment. In this paper, we put forward a view of directly utilizing the spatial structure size of the AS1411 aptamer as the nano-gatekeeper on the pore openings of MCM-41 type mesoporous silica and thus constructed a redox-responsive drug delivery system named MCM-41-SS-AS1411. The particles obtained at each step were characterized by TEM, FTIR, SXRD, TGA and zeta potential measurement. The characterization data confirmed that the particles were successfully prepared. The binding amount of the aptamer was 3.1 × 10 for each carrier particle averagely. The anticancer drug Dox was regarded as a drug model to investigate the redox-controlled drug release behavior by fluorescence measurements. The investigation results demonstrate that the spatial volume of aptamer AS1411 can block the mesopore, and this drug-carrier can realize controlled drug release by GSH. We hope this idea can play a prompt role in relevant research. Meanwhile, the preparation steps of this DDS are simplified.
近年来，多种氧化还原响应型药物递送系统应运而生，旨在防止药物过早释放并降低其在癌症治疗中对人体的毒性。在本文中，我们提出了一种观点，即直接利用AS1411适配体的空间结构尺寸作为MCM-41型介孔二氧化硅孔口处的纳米守门人，从而构建了一种名为MCM-41-SS-AS1411的氧化还原响应型药物递送系统。通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、小角X射线衍射（SXRD）、热重分析（TGA）和zeta电位测量对每一步获得的颗粒进行了表征。表征数据证实颗粒已成功制备。每个载体颗粒上适配体的平均结合量为3.1×10。以抗癌药物阿霉素作为药物模型，通过荧光测量研究氧化还原控制的药物释放行为。研究结果表明，适配体AS1411的空间体积可以堵塞介孔，并且这种药物载体可以通过谷胱甘肽实现药物的控释。我们希望这一想法能在相关研究中起到推动作用。同时，该药物递送系统的制备步骤得到了简化。
