Buono Sean A, Godwin Hilary A, Green Nicole M
Department of Environmental Health Sciences, Fielding School of Public Health, University of California, Los Angeles, CA 90095; Los Angeles County Public Health Laboratories, Los Angeles County, Department of Public Health, Downey, CA 90242.
Department of Environmental Health Sciences, Fielding School of Public Health, University of California, Los Angeles, CA 90095; Institute of the Environment and Sustainability, University of California, Los Angeles, CA 90095.
Diagn Microbiol Infect Dis. 2017 Nov;89(3):173-177. doi: 10.1016/j.diagmicrobio.2017.07.008. Epub 2017 Jul 26.
We compared the performance and ease of use for three high-throughput treponemal immunoassays: Phoenix Biotech Trep-Sure Total Antibody EIA, Siemens ADVIA® Centaur Syphilis Assay, and DiaSorin LIAISON® Treponema Assay. One thousand serum samples submitted for routine screening were used in this study. Each assay demonstrated comparable sensitivity, specificity, and percent agreement (98-100%) compared with Treponema pallidum particle agglutination (TP-PA). Thus, treponemal immunoassays are an acceptable alternative for syphilis screening or confirmatory testing. Batch sizes and technologist active time varied between each treponemal immunoassay; the chemiluminescence platforms offered significantly greater ability to batch (random access vs. fixed batch sizes) in less time. When we compared the results obtained using a reverse algorithm approach to those obtained using a traditional algorithm, we found that the reverse algorithm identified 38 additional seropositive individuals that were not detected using the traditional algorithm. Clinical evaluation was useful for resolving cases with discordant serology.
凤凰生物科技Trep-Sure总抗体酶免疫测定法、西门子ADVIA®Centaur梅毒检测法和索林集团LIAISON®梅毒螺旋体检测法。本研究使用了1000份提交进行常规筛查的血清样本。与梅毒螺旋体颗粒凝集试验（TP-PA）相比，每种检测方法都显示出相当的敏感性、特异性和一致性百分比（98-100%）。因此，梅毒螺旋体免疫测定法是梅毒筛查或确证试验的可接受替代方法。每种梅毒螺旋体免疫测定法的批次大小和技术人员操作时间各不相同；化学发光平台在更短的时间内提供了显著更强的批量检测能力（随机进样与固定批次大小）。当我们将使用反向算法方法获得的结果与使用传统算法获得的结果进行比较时，我们发现反向算法识别出了另外38例使用传统算法未检测到的血清学阳性个体。临床评估有助于解决血清学结果不一致的病例。
