Zhang Cong, Liu Shou-Jin, Yang Liu, Yuan Ming-Yan, Li Jin-Yu, Hou Bo, Li Hong-Mei, Yang Xing-Zhi, Ding Chang-Chun, Hu Jiang-Miao
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China; School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, People's Republic of China.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, People's Republic of China.
Fitoterapia. 2017 Oct;122:76-79. doi: 10.1016/j.fitote.2017.08.015. Epub 2017 Aug 24.
A new bibenzyl derivative, dendrocandin V (1) and a new sesquiterpene amino ether, wardianumine A (2), together with eleven known compounds, including phenanthrenes (denbinobin (3), 9,10-dihydro-denbinobin (4), mostatin (5), loddigesiinols A (6)), bibenzyls (moscatilin (7), 5-hydroxy-3,4'-dimethoxybibenzyl (8), 3,4-dihydroxy-5,4'-dimethoxy bibenzyl (9), dendrocandin A (10), gigantol (11), dendrocandin U (12)) and an alkaloids (dihydroshihunine, 13) were isolated from the EtOH extraction of stems of Dendrobium wardianum Warner. Isolation of the new compound 2 indicated that N,N-dimethylethanolamine as the key adduction in the synthesis of dendroxine and its analogs in Dendrobium species. The hypothetical biosynthetic pathway of 2 was then postulated. Inspired by literature and traditional usage of the herbal medicine, some compounds were sent for cytotoxic activity and the results indicated that compounds 1, 3, 4, 5 showed cytotoxic activities against five human cancer cell lines (HL-60, A-549, SMMC-7721, MCF-7, and SW-480) with IC50 from 2.33-38.48μM. Among those compounds, 3 and 4 showed cell line selectivity with strong activity comparable to DDP.
从华石斛茎的乙醇提取物中分离出一种新的联苄衍生物石仙桃素V（1）、一种新的倍半萜氨基醚华石斛胺A（2），以及11种已知化合物，包括菲类化合物（石蒜碱（3）、9,10-二氢石蒜碱（4）、莫司他汀（5）、洛迪吉西醇A（6））、联苄类化合物（香石蒜醌（7）、5-羟基-3,4'-二甲氧基联苄（8）、3,4-二羟基-5,4'-二甲氧基联苄（9）、石仙桃素A（10）、石斛酚（11）、石仙桃素U（12））和一种生物碱（二氢石胡宁，13）。新化合物2的分离表明N,N-二甲基乙醇胺是石斛属植物中石斛碱及其类似物合成中的关键加成物。随后推测了2的假设生物合成途径。受文献和该草药传统用途的启发，对一些化合物进行了细胞毒性活性测试，结果表明化合物1、3、4、5对五种人类癌细胞系（HL-60、A-549、SMMC-7721、MCF-7和SW-480）具有细胞毒性活性，IC50为2.33 - 38.48μM。在这些化合物中，3和4表现出细胞系选择性，其强活性与顺铂相当。
