Kovacs Bernadett, Zomborszki Zoltan Peter, Orban-Gyapai Orsolya, Csupor-Loffler Boglarka, Liktor-Busa Erika, Lazar Andrea, Papp Viktor, Urban Edit, Hohmann Judit, Vanyolos Attila
Department of Pharmacognosy, University of Szeged, Szeged, Hungary.
Institute of Clinical Microbiology, University of Szeged, Szeged, Hungary.
Int J Med Mushrooms. 2017;19(5):387-394. doi: 10.1615/IntJMedMushrooms.v19.i5.10.
Ten representative Central European phellinoid Hymenochaetaceae species (Phellinus sensu lato) were selected to examine their potential pharmacological activity. In this study 40 organic (n-hexane, chloroform, 50% methanol) and aqueous extracts with different polarities were analyzed for their antimicrobial, antioxidant, and xanthine oxidase (XO)--inhibitory properties. Fomitiporia robusta, Fuscoporia torulosa, Phellopilus nigrolimitatus, and Porodaedalea chrysoloma showed moderate antibacterial activity; Bacillus subtilis ATCC 6633, methicillin-resistant Staphylococcus aureus ATCC 43300, and Moraxella catarrhalis ATCC 43617 were the strains most susceptible to the examined fungal species. The in vitro antioxidant and XO assays demonstrated that most of the selected species possess remarkable antioxidant and XO-inhibitory activities. The water extracts in general proved to be more active antioxidants than organic extracts. In the case of F. torulosa, Ph. Nigrolimitatus, and P. chrysoloma, the results of DPPH tests correlate well with those obtained by oxygen radical absorbance capacity tests; these mushrooms presented high antioxidant activities in both assays. Future studies involving phellinoid Hymenochaetaceae species are planned, which may furnish novel results in terms of the species' pharmacological activity and the specific compounds responsible for the observed activity.
选取了10种具有代表性的中欧拟层孔菌科层孔菌属（广义桑黄属）物种，以研究它们潜在的药理活性。在本研究中，分析了40种不同极性的有机提取物（正己烷、氯仿、50%甲醇）和水提取物的抗菌、抗氧化及黄嘌呤氧化酶（XO）抑制特性。粗壮拟层孔菌、圆瘤褐层孔菌、黑边拟层孔菌和金黄拟层孔菌表现出中等抗菌活性；枯草芽孢杆菌ATCC 6633、耐甲氧西林金黄色葡萄球菌ATCC 43300和卡他莫拉菌ATCC 43617是对所检测真菌物种最敏感的菌株。体外抗氧化和XO检测表明，大多数所选物种具有显著的抗氧化和XO抑制活性。总体而言，水提取物被证明比有机提取物具有更强的抗氧化活性。对于圆瘤褐层孔菌、黑边拟层孔菌和金黄拟层孔菌，DPPH测试结果与氧自由基吸收能力测试结果相关性良好；在这两种检测中，这些蘑菇均呈现出高抗氧化活性。计划开展涉及拟层孔菌科层孔菌属物种的后续研究，这可能会在物种的药理活性以及导致观察到的活性的特定化合物方面提供新的结果。
