一种改进的ATAC-seq方案可减少背景干扰，并能够对冷冻组织进行检测。
An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues.
作者信息
Corces M Ryan, Trevino Alexandro E, Hamilton Emily G, Greenside Peyton G, Sinnott-Armstrong Nicholas A, Vesuna Sam, Satpathy Ansuman T, Rubin Adam J, Montine Kathleen S, Wu Beijing, Kathiria Arwa, Cho Seung Woo, Mumbach Maxwell R, Carter Ava C, Kasowski Maya, Orloff Lisa A, Risca Viviana I, Kundaje Anshul, Khavari Paul A, Montine Thomas J, Greenleaf William J, Chang Howard Y
机构信息
Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
Department of Dermatology, Stanford University School of Medicine, Stanford, California, USA.
出版信息
Nat Methods. 2017 Oct;14(10):959-962. doi: 10.1038/nmeth.4396. Epub 2017 Aug 28.
Abstract
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.
摘要
我们展示了Omni-ATAC，这是一种经过改进的用于染色质可及性分析的ATAC-seq方案，它适用于多种应用，显著提高了信噪比和信息含量。Omni-ATAC方案可从存档的冷冻组织样本和50微米切片生成染色质可及性图谱，揭示不同人类脑结构中与疾病相关的DNA元件的活性。Omni-ATAC方案能够在组织背景和转化研究中对个体调控组进行研究。
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