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鉴定出28个能检测5号染色体短臂13个不同物理区域中限制性片段长度多态性（RFLP）的DNA片段。

Identification of 28 DNA fragments that detect RFLPs in 13 distinct physical regions of the short arm of chromosome 5.

作者信息

Overhauser J, McMahan J, Wasmuth J J

出版信息

Nucleic Acids Res. 1987 Jun 11;15(11):4617-27. doi: 10.1093/nar/15.11.4617.

DOI:10.1093/nar/15.11.4617
PMID:2884625
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340884/
Abstract

A series of 175 lambda phage carrying human inserts isolated from a library that is specific for the short arm of human chromosome 5 (5p) have been regionally mapped on 5p using a deletion mapping panel of 16 human-hamster cell hybrids, each of which contains a chromosome 5 with a different deletion in the short arm. Seventy-five single copy DNA fragments were screened with 12 restriction enzymes for their ability to detect restriction fragment length polymorphisms (RFLPs). Twenty-eight of these DNA fragments, which are located in 13 distinct physical regions of 5p, were found to detect RFLPs. These DNA markers make it possible to construct a linkage map that will span the entire length of 5p and will allow the relationship between genetic and physical distance for this region of the genome to be examined at a high level of resolution.

摘要

从一个针对人类5号染色体短臂（5p）的文库中分离出一系列携带人类插入片段的175个λ噬菌体，利用由16个人-仓鼠细胞杂种组成的缺失作图板，将它们区域定位在5p上，每个杂种细胞都含有一条5号染色体，其短臂有不同的缺失。用12种限制酶筛选75个单拷贝DNA片段，检测其检测限制片段长度多态性（RFLP）的能力。这些DNA片段中有28个位于5p的13个不同物理区域，被发现能检测到RFLP。这些DNA标记使得构建一个跨越5p全长的连锁图谱成为可能，并将使基因组该区域遗传距离与物理距离之间的关系得以在高分辨率水平上进行研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/340884/89110548a75d/nar00255-0259-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/340884/6cc79a42a694/nar00255-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/340884/7cb1f19a157f/nar00255-0259-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/340884/89110548a75d/nar00255-0259-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/340884/6cc79a42a694/nar00255-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/340884/7cb1f19a157f/nar00255-0259-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d047/340884/89110548a75d/nar00255-0259-b.jpg

相似文献

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本文引用的文献

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Linkage of the leuS, emtB, and chr genes on chromosome 5 in humans and expression of human genes encoding protein synthetic components in human--Chinese hamster hybrids.人类5号染色体上leuS、emtB和chr基因的连锁以及人类-中国仓鼠杂交细胞中编码蛋白质合成成分的人类基因的表达。
Somatic Cell Genet. 1982 Mar;8(2):245-64. doi: 10.1007/BF01538680.
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A highly polymorphic locus in human DNA.人类DNA中的一个高度多态性位点。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6754-8. doi: 10.1073/pnas.77.11.6754.
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J Cell Biol. 1994 Mar;124(6):871-82. doi: 10.1083/jcb.124.6.871.
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Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.可通过聚合酶链反应进行分型的大量人类DNA多态性类别。
Am J Hum Genet. 1989 Mar;44(3):388-96.
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Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.人苏氨酰 - tRNA合成酶cDNA克隆的分离：抗硼霉素细胞系中结构基因的扩增
Mol Cell Biol. 1989 May;9(5):1832-8. doi: 10.1128/mcb.9.5.1832-1838.1989.
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Prenatal diagnosis and carrier detection of a cryptic translocation by using DNA markers from the short arm of chromosome 5.利用5号染色体短臂的DNA标记进行隐匿性易位的产前诊断和携带者检测。
Am J Hum Genet. 1989 Aug;45(2):296-303.
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Expression and chromosomal localization of a lymphocyte K+ channel gene.一种淋巴细胞钾离子通道基因的表达及染色体定位
Proc Natl Acad Sci U S A. 1990 Dec;87(23):9411-5. doi: 10.1073/pnas.87.23.9411.
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Linkage analysis in spinal muscular atrophy, by six closely flanking markers on chromosome 5.利用位于5号染色体上的六个紧密连锁的标记对脊髓性肌萎缩症进行连锁分析。
Am J Hum Genet. 1991 Apr;48(4):764-8.
9
A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Déjérine) muscular dystrophy: linkage analysis of six autosomes.一组临床特征相同的面肩肱型（兰杜济 - 德热里纳型）肌营养不良症家族：六条常染色体的连锁分析
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10
Amerindian mitochondrial DNAs have rare Asian mutations at high frequencies, suggesting they derived from four primary maternal lineages.美洲印第安人的线粒体DNA有罕见的亚洲突变，且频率很高，这表明它们源自四个主要的母系谱系。
Am J Hum Genet. 1990 Mar;46(3):613-23.
一种将DNA限制性内切酶片段放射性标记至高比活度的技术。附录
Anal Biochem. 1984 Feb;137(1):266-7. doi: 10.1016/0003-2697(84)90381-6.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
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7
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10
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