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人苏氨酰 - tRNA合成酶cDNA克隆的分离：抗硼霉素细胞系中结构基因的扩增

Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.

作者信息

Kontis K J, Arfin S M

机构信息

Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717.

出版信息

Mol Cell Biol. 1989 May;9(5):1832-8. doi: 10.1128/mcb.9.5.1832-1838.1989.

DOI:10.1128/mcb.9.5.1832-1838.1989
PMID:2747635
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362973/
Abstract

A cDNA for threonyl-tRNA synthetase was isolated from a human placental cDNA lambda gt11 expression library by immunological screening, and its identity was confirmed by hybrid-selected mRNA translation. With this cDNA used as a hybridization probe, borrelidin-resistant Chinese hamster ovary cells that overproduced threonyl-tRNA synthetase were shown to have increased levels of threonyl-tRNA synthetase mRNA and gene sequences. Amplification of the gene did not appear to have been accompanied by any major structural reorganizations.

摘要

通过免疫筛选从人胎盘cDNA λgt11表达文库中分离出苏氨酰-tRNA合成酶的cDNA，并通过杂交选择的mRNA翻译确认其身份。以该cDNA作为杂交探针，结果显示，过量产生苏氨酰-tRNA合成酶的抗硼relidin中国仓鼠卵巢细胞中，苏氨酰-tRNA合成酶mRNA和基因序列水平升高。该基因的扩增似乎并未伴随任何主要的结构重组。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/4536c0c6594d/molcellb00053-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/94b3d7c60b75/molcellb00053-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/f5d47990eaa2/molcellb00053-0032-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/c1df19a74154/molcellb00053-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/1c92493dd402/molcellb00053-0033-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/7c9d51e81190/molcellb00053-0033-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/7e5fa8ed4b7e/molcellb00053-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/b562d5ac609a/molcellb00053-0034-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/4536c0c6594d/molcellb00053-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/94b3d7c60b75/molcellb00053-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/f5d47990eaa2/molcellb00053-0032-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/c1df19a74154/molcellb00053-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/1c92493dd402/molcellb00053-0033-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/7c9d51e81190/molcellb00053-0033-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/7e5fa8ed4b7e/molcellb00053-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/b562d5ac609a/molcellb00053-0034-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f98/362973/4536c0c6594d/molcellb00053-0035-a.jpg

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Isolation of a cDNA clone for human threonyl-tRNA synthetase: amplification of the structural gene in borrelidin-resistant cell lines.人苏氨酰 - tRNA合成酶cDNA克隆的分离：抗硼霉素细胞系中结构基因的扩增
Mol Cell Biol. 1989 May;9(5):1832-8. doi: 10.1128/mcb.9.5.1832-1838.1989.
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Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line.在一株对硼雷素耐药的中国仓鼠卵巢细胞系中苏氨酰-tRNA合成酶水平升高。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5367-70. doi: 10.1073/pnas.78.9.5367.
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Chinese hamster ovary cells resistant to borrelidin overproduce threonyl-tRNA synthetase.对硼霉素具有抗性的中国仓鼠卵巢细胞过量产生苏氨酰 - tRNA合成酶。
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引用本文的文献

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Hum Genet. 1990 Oct;85(5):527-30. doi: 10.1007/BF00194231.
2
Evidence that gene G7a in the human major histocompatibility complex encodes valyl-tRNA synthetase.人类主要组织相容性复合体中的基因G7a编码缬氨酰-tRNA合成酶的证据。
Biochem J. 1991 Sep 15;278 ( Pt 3)(Pt 3):809-16. doi: 10.1042/bj2780809.

本文引用的文献

1
Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line.在一株对硼雷素耐药的中国仓鼠卵巢细胞系中苏氨酰-tRNA合成酶水平升高。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5367-70. doi: 10.1073/pnas.78.9.5367.
2
Chinese hamster ovary cells resistant to borrelidin overproduce threonyl-tRNA synthetase.对硼霉素具有抗性的中国仓鼠卵巢细胞过量产生苏氨酰 - tRNA合成酶。
J Biol Chem. 1984 Jul 25;259(14):9202-6.
3
Threonyl-tRNA synthetase from Chinese hamster ovary cells is phosphorylated on serine.来自中国仓鼠卵巢细胞的苏氨酰-tRNA合成酶在丝氨酸上被磷酸化。
J Biol Chem. 1984 Sep 25;259(18):11160-1.
4
Specific sequence homology and three-dimensional structure of an aminoacyl transfer RNA synthetase.氨酰基转移RNA合成酶的特定序列同源性和三维结构
Science. 1984 Dec 14;226(4680):1315-7. doi: 10.1126/science.6390679.
5
Gene amplification in cultured animal cells.培养动物细胞中的基因扩增。
Cell. 1984 Jul;37(3):705-13. doi: 10.1016/0092-8674(84)90406-9.
6
Yeast RNA polymerase II genes: isolation with antibody probes.酵母RNA聚合酶II基因：用抗体探针分离
Science. 1983 Nov 18;222(4625):778-82. doi: 10.1126/science.6356359.
7
Diadenosine 5', 5"'-P1, P4-tetraphosphate: a pleiotropically acting alarmone?5',5'''-P1,P4-四磷酸二腺苷：一种具有多效作用的警报素？
Cell. 1983 Oct;34(3):711-2. doi: 10.1016/0092-8674(83)90526-3.
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Diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A): its role in cellular metabolism.二腺苷5',5"'-P1,P4-四磷酸（Ap4A）：其在细胞代谢中的作用。
Anal Biochem. 1983 Oct 1;134(1):1-10. doi: 10.1016/0003-2697(83)90255-5.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
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Efficient isolation of genes by using antibody probes.使用抗体探针高效分离基因。
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8. doi: 10.1073/pnas.80.5.1194.