Nyberg F, Nylander I
Regul Pept. 1987 Mar;17(3):159-66. doi: 10.1016/0167-0115(87)90025-5.
Dynorphin A immunoreactivity in human cerebrospinal fluid has been characterized. Large quantities of the fluid were fractionated by molecular sieving on a Sephadex G-50 column and analyzed by radioimmunoassay. Active fractions were further analyzed by means of HPLC and an enzyme radioimmunoassay procedure for identification of Leu-enkephalin-Arg6 sequences. Several dynorphin A-active components of varying sizes were identified. However, most immunoreactive material derived from species of higher molecular weight (Mr 3000 and 5000) than authentic dynorphin A (Mr 2000). The Leu-enkephalin core was found to reside in both these large structures.
人体脑脊液中强啡肽A免疫反应性已得到鉴定。通过在葡聚糖G - 50柱上进行分子筛对大量脑脊液进行分级分离，并通过放射免疫分析进行分析。活性级分通过高效液相色谱法和酶放射免疫分析程序进一步分析，以鉴定亮脑啡肽 - Arg6序列。鉴定出了几种大小不同的强啡肽A活性成分。然而，大多数免疫反应性物质来源于分子量（Mr 3000和5000）高于正宗强啡肽A（Mr 2000）的物种。发现亮脑啡肽核心存在于这两种大结构中。
