Tozawa F, Suda T, Tachibana S, Tomori N, Demura H, Shizume K
Life Sci. 1984 Oct 15;35(16):1633-7. doi: 10.1016/0024-3205(84)90173-5.
Immunoreactive dynorphin (I-dynorphin) was measured by radioimmunoassay in human cerebrospinal fluid (CSF). I-dynorphin concentration in CSF was 30 +/- 2 pg/ml. Sephadex G-25 column chromatography showed the main peak eluted at the position of dynorphin-(1-17). HPLC elution profile of this major peak from gel filtration showed a large peak corresponding to the position of dynorphin-(1-17) and small peaks corresponding to the positions of dynorphin-(1-13), dynorphin-(1-12) and other unknown peptides.
采用放射免疫分析法测定人脑脊液(CSF)中的免疫反应性强啡肽(I-强啡肽)。脑脊液中I-强啡肽浓度为30±2皮克/毫升。葡聚糖凝胶G-25柱色谱显示主要峰在强啡肽-(1-17)位置洗脱。该凝胶过滤主要峰的高效液相色谱洗脱图谱显示对应强啡肽-(1-17)位置的大峰以及对应强啡肽-(1-13)、强啡肽-(1-12)和其他未知肽位置的小峰。