Tawfike Ahmed F, Abbott Grainne, Young Louise, Edrada-Ebel RuAngelie
Strathclyde Institute of Pharmacy and Biomedical Science, University of Strathclyde, United Kingdom.
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Egypt.
Planta Med. 2018 Feb;84(3):182-190. doi: 10.1055/s-0043-118807. Epub 2017 Aug 28.
Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints of solid and liquid culture extracts of endophyte sp. isolated from the leaves of . Natural product databases were used to dereplicate metabolites in order to determine known compounds and the presence of new natural products. Multivariate analysis highlighted the putative metabolites responsible for the bioactivity of the fungal extract and its fractions on NF-B and the myelogenous leukemia cell line K562. Metabolomic tools and dereplication studies using high-resolution electrospray ionization mass spectrometry directed the fractionation and isolation of the bioactive components from the fungal extracts. This resulted in the isolation of -acetylphenylalanine (1: ) and two linear peptide congeners of 1: : dipeptide -acetylphenylalanyl-L-phenylalanine (2: ) and tripeptide -acetylphenylalanyl-L-phenylalanyl-L-leucine (3: ).
与药用植物相关的内生真菌是新化学物质和生物学特性的潜在来源。代谢组学工具已成功用于比较从[植物名称]叶片中分离出的内生菌[内生菌名称]固体和液体培养提取物的代谢物指纹图谱。利用天然产物数据库对代谢物进行去重复分析，以确定已知化合物和新天然产物的存在。多变量分析突出了负责真菌提取物及其馏分对核因子-κB（NF-κB）和髓性白血病细胞系K562生物活性的推定代谢物。代谢组学工具和使用高分辨率电喷雾电离质谱的去重复研究指导了从真菌提取物中分离生物活性成分。这导致分离出了α-乙酰苯丙氨酸（1：[具体结构]）和1的两个线性肽同系物：二肽α-乙酰苯丙氨酰-L-苯丙氨酸（2：[具体结构]）和三肽α-乙酰苯丙氨酰-L-苯丙氨酰-L-亮氨酸（3：[具体结构]）。
