Department of Biotechnology, Thapar Institute of Engineering & Technology, Patiala, Punjab, 147004, India.
World J Microbiol Biotechnol. 2019 May 3;35(5):74. doi: 10.1007/s11274-019-2651-8.
In the present study, an endophytic fungal strain was isolated from its non-Taxus host plant Terminalia arjuna and identified as Alternaria brassicicola based on its morphological characteristics and internal transcribed spacer sequence analysis. This fungus was grown in potato dextrose broth and analyzed for the presence of taxol by using chromatographic and spectrometric techniques. The ethyl acetate extract of A.brassicicola was subjected to column chromatography. Among the different fractions, the fraction 7 showed positive to taxol, which was further confirmed by UV absorption, HPLC, FTIR spectra and LC-ESI-MS by comparing with the authentic taxol (Paclitaxel). The peaks of fraction 7 obtained by UV spectroscopy, FTIR and HPLC analysis were quite similar to that of standard taxol confirming the presence of taxol. A parent ion peak of m/z 854.95 was observed in the LC-ESI-MS spectrum which was similar to paclitaxel with reported m/z of 854 [M+H] ion. A. brassicicola produced about 140.8 μg/l taxol as quantified through HPLC. Present study results suggest that the endophytic fungus A.brassicicola serves as a potential source for the production of taxol isolated from non-Taxus plant.
在本研究中，从非紫杉宿主植物诃子中分离出一株内生真菌，并根据其形态特征和内部转录间隔区序列分析鉴定为交链格孢菌。该真菌在土豆葡萄糖肉汤中生长，并通过色谱和光谱技术分析其紫杉醇的存在。交链格孢菌的乙酸乙酯提取物经柱层析分离。在不同的馏分中，馏分 7 显示出对紫杉醇的阳性反应，通过与紫杉醇（紫杉醇）的紫外吸收、HPLC、FTIR 光谱和 LC-ESI-MS 比较进一步证实。通过紫外光谱、FTIR 和 HPLC 分析得到的馏分 7 的峰与标准紫杉醇非常相似，证实了紫杉醇的存在。LC-ESI-MS 谱中观察到母离子峰 m/z 854.95，与报道的 m/z 854 [M+H]+离子的紫杉醇相似。HPLC 定量检测到交链格孢菌产生约 140.8μg/l 的紫杉醇。本研究结果表明，内生真菌交链格孢菌是从非紫杉植物中分离出紫杉醇的潜在来源。
