Wang Zhiyong, Gao Kuo, Xu Can, Gao Jian, Yan Yujing, Wang Yingfeng, Li Zhongfeng, Chen Jianxin
Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Beijing University of Chinese Medicine, Beijing, 100029, China.
Magn Reson Chem. 2018 Jan;56(1):5-17. doi: 10.1002/mrc.4658. Epub 2017 Sep 12.
Hedyotis diffusa, a traditional Chinese herbal medicine, is widely used for oncotherapy and shows a positive effect in the clinical treatment. But its mechanism of anticancer activities is complicated and unclear. This study was undertaken to assess the therapeutic effects and reveal detailed mechanisms of H. diffusa for oncotherapy. A Walker 256 tumor-bearing rat model was established, and metabolomic profiles of plasma and urine were obtained from H NMR technique. Multivariate statistical analysis methods were used to characterize the discriminating metabolites between control (C), Walker 256 tumor-bearing rats model (M), and H. diffusa treatment (H) groups. Finally, 13 and 10 metabolomic biomarkers in urine and plasma samples were further identified as characteristic metabolites in M group, whereas H group showed a partial metabolic balance recovered, such as ornithine, N-acetyl-l-aspartate, l-aspartate, and creatinine in urine samples, and acetate, lactate, choline, l-glutamine, and 3-hydroxybutyrate in plasma samples. On the basis of the methods above, we hypothesized H. diffusa treatment reduced the injury caused by Walker 256 tumor and maintained a metabolic balance. Our study demonstrated that this method provided new insights into metabolic alterations in tumor-bearing biosystems and researching on the effects of H. diffusa on the endogenous metabolism in tumor-bearing rats.
白花蛇舌草是一种传统的中草药，广泛用于肿瘤治疗，在临床治疗中显示出积极效果。但其抗癌活性机制复杂且尚不清楚。本研究旨在评估白花蛇舌草对肿瘤治疗的效果并揭示其详细机制。建立了Walker 256荷瘤大鼠模型，采用核磁共振技术获取血浆和尿液的代谢组学图谱。运用多元统计分析方法对对照组（C）、Walker 256荷瘤大鼠模型组（M）和白花蛇舌草治疗组（H）之间的差异代谢物进行表征。最终，尿液和血浆样本中的13种和10种代谢组学生物标志物被进一步鉴定为M组的特征性代谢物，而H组显示出部分代谢平衡得以恢复，如尿液样本中的鸟氨酸、N-乙酰-L-天冬氨酸、L-天冬氨酸和肌酐，以及血浆样本中的乙酸盐、乳酸盐、胆碱、L-谷氨酰胺和3-羟基丁酸盐。基于上述方法，我们推测白花蛇舌草治疗可减轻Walker 256肿瘤造成的损伤并维持代谢平衡。我们的研究表明，该方法为荷瘤生物系统中的代谢改变以及白花蛇舌草对荷瘤大鼠内源性代谢影响的研究提供了新的见解。
