Wazir Junaid, Ullah Rahat, Khongorzul Puregmaa, Hossain Md Amir, Khan Muhammad Waqar, Aktar Nasrin, Cui Xingxing, Zhou Xiaohui
Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, China.
Antibody Engineering Laboratory, School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu, China.
Andrologia. 2021 Feb;53(1):e13913. doi: 10.1111/and.13913. Epub 2020 Nov 24.
Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a frustrating and often debilitating disease. Current studies have shown that Traditional Chinese Medicine (TCM) can improve patients' quality of life and alleviate CP/CPPS symptoms. In this study, the efficacy of Hedyotis diffusa Willd aqueous extraction in experimental autoimmune prostatitis (EAP) mice models was revealed. The C57BL/6 mice were randomly assigned to three groups. Except for the control group, all other groups were subcutaneously injected with 0.2 ml emulsion of T2 peptide, on day 0 and day 14, for inducing EAP models. After the EAP modelling, oral saline was given to the model group, while the H. diffusa group was treated with aqueous extract of H. diffusa Willd. Micturition habits and withdrawal response frequencies were measured. Haematoxylin and eosin staining and immunohistochemistry were used to investigate inflammatory cell infiltration and TNF-α in the prostate tissue respectively. TNF-α levels in the serum were evaluated by ELISA. The H. diffusa Willd aqueous extraction considerably reduced the urine spots number and increased the pain threshold in H. diffusa group. H. diffusa group showed significantly reduced inflammatory lesion and inflammatory cell infiltration than the model group. The levels of TNF-α in H. diffusa group were considerably reduced.
慢性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）是一种令人沮丧且常常使人虚弱的疾病。目前的研究表明，中医可以改善患者的生活质量并缓解CP/CPPS症状。在本研究中，揭示了白花蛇舌草水提取物在实验性自身免疫性前列腺炎（EAP）小鼠模型中的疗效。将C57BL/6小鼠随机分为三组。除对照组外，所有其他组在第0天和第14天皮下注射0.2 ml T2肽乳剂，以诱导EAP模型。EAP建模后，模型组给予口服生理盐水，而白花蛇舌草组用白花蛇舌草水提取物治疗。测量排尿习惯和撤药反应频率。分别采用苏木精-伊红染色和免疫组织化学法研究前列腺组织中的炎性细胞浸润和TNF-α情况。通过酶联免疫吸附测定法评估血清中TNF-α水平。白花蛇舌草水提取物显著减少了白花蛇舌草组小鼠的尿斑数量并提高了疼痛阈值。与模型组相比，白花蛇舌草组的炎性病变和炎性细胞浸润明显减少。白花蛇舌草组的TNF-α水平显著降低。
