Morrison Ashby J
Department of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA
Philos Trans R Soc Lond B Biol Sci. 2017 Oct 5;372(1731). doi: 10.1098/rstb.2016.0289.
Chromatin modification is conserved in all eukaryotes and is required to facilitate and regulate DNA-templated processes. For example, chromatin manipulation, such as histone post-translational modification and nucleosome positioning, play critical roles in genome stability pathways. The INO80 chromatin-remodelling complex, which regulates the abundance and positioning of nucleosomes, is particularly important for proper execution of inducible responses to DNA damage. This review discusses the participation and activity of the INO80 complex in DNA repair and cell cycle checkpoint pathways, with emphasis on the model system. Furthermore, the role of ATM/ATR kinases, central regulators of DNA damage signalling, in the regulation of INO80 function will be reviewed. In addition, emerging themes of chromatin remodelling in mitotic stability pathways and chromosome segregation will be introduced. These studies are critical to understanding the dynamic chromatin landscape that is rapidly and reversibly modified to maintain the integrity of the genome.This article is part of the themed issue 'Chromatin modifiers and remodellers in DNA repair and signalling'.
染色质修饰在所有真核生物中都是保守的，并且是促进和调节DNA模板化过程所必需的。例如，染色质操纵，如组蛋白翻译后修饰和核小体定位，在基因组稳定性途径中发挥着关键作用。INO80染色质重塑复合物可调节核小体的丰度和定位，对正确执行DNA损伤诱导反应尤为重要。本综述讨论了INO80复合物在DNA修复和细胞周期检查点途径中的参与和活性，重点是模型系统。此外，还将综述DNA损伤信号的核心调节因子ATM/ATR激酶在INO80功能调节中的作用。此外，还将介绍有丝分裂稳定性途径和染色体分离中染色质重塑的新主题。这些研究对于理解动态染色质景观至关重要，该景观会迅速且可逆地发生修饰以维持基因组的完整性。本文是主题为“DNA修复和信号传导中的染色质修饰剂和重塑剂”的特刊的一部分。
