Department of Gynecology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Mol Med Rep. 2017 Oct;16(4):5499-5504. doi: 10.3892/mmr.2017.7282. Epub 2017 Aug 18.
Aquaporin 1 (AQP1), which is a water channel protein, has been demonstrated to have an important role in cell proliferation and migration of various cancers. However, its specific role in ovarian cancer remains to be elucidated. The present study demonstrated that AQP1 expression was elevated in the majority of patients with ovarian cancer compared with normal ovarian tissues. In addition, a short interfering (si)RNA targeting AQP1 was established, and transfected into the SKOV3 ovarian cancer cell line, to investigate the effects on cell viability, apoptosis, migration and invasion in the ovarian cancer cells using an MTT assay, flow cytometry, wound healing and Transwell invasion chamber assays, respectively. The results of the present study demonstrated that siRNA targeting AQP1 effectively downregulated AQP1 expression at the mRNA and protein levels, markedly suppressed cell viability, migration and invasion and promoted apoptosis of ovarian cancers cells. These results suggested that AQP1 may serve as a novel target for ovarian cancer treatment in the future.
水通道蛋白 1（AQP1）是一种水通道蛋白，已被证明在各种癌症的细胞增殖和迁移中具有重要作用。然而，其在卵巢癌中的具体作用仍有待阐明。本研究表明，与正常卵巢组织相比，大多数卵巢癌患者的 AQP1 表达升高。此外，还构建了针对 AQP1 的短发夹 RNA（siRNA），并转染至 SKOV3 卵巢癌细胞系，分别采用 MTT 检测、流式细胞术、划痕愈合实验和 Transwell 侵袭实验，研究其对卵巢癌细胞活力、凋亡、迁移和侵袭的影响。本研究结果表明，靶向 AQP1 的 siRNA 可有效下调 AQP1 在 mRNA 和蛋白水平的表达，显著抑制卵巢癌细胞活力、迁移和侵袭，并促进细胞凋亡。这些结果表明，AQP1 可能成为未来卵巢癌治疗的新靶点。
