Ji Yinan, Liao Xiaoming, Jiang Yi, Wei Wei, Yang Huawei
Department of Breast Surgery, Guangxi Medical University Cancer Hospital, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.
Oncol Lett. 2021 Jun;21(6):437. doi: 10.3892/ol.2021.12698. Epub 2021 Apr 1.
Aquaporin 1 (AQP1) contributes to the progression of several cancer types, but its potential involvement in triple-negative breast cancer (TNBC) is unclear. The aim of the present study was to examine the role of AQP1 in cell proliferation and invasion in TNBC. Reverse transcription-quantitative PCR analysis and western blotting were used to detect AQP1 expression in different cell lines. A short hairpin (sh)RNA targeting AQP1 was established and transfected into MDA-MB-231 breast cancer cells. To investigate the effects of AQP1 knockdown, breast cancer cell proliferation, migration and invasion were evaluated by Cell Counting Kit-8 and Transwell assays. Furthermore, the volume and weight of tumor xenografts in mice were measured to evaluate breast cancer growth ability. The results revealed that the levels of AQP1 were higher in the MDA-MB-231 cell line compared with those in other breast cancer cell lines (MCF-7 and SK-BR-3) and a normal mammary epithelial cell line (MCF-10A). The shRNA targeting AQP1 effectively downregulated AQP1 expression at the mRNA and protein levels, and markedly suppressed TNBC cell proliferation, migration and invasion , and tumor growth . These results suggested that AQP1 may serve as a potential therapeutic target in TNBC.
水通道蛋白1（AQP1）促进多种癌症类型的进展，但其在三阴性乳腺癌（TNBC）中的潜在作用尚不清楚。本研究旨在探讨AQP1在TNBC细胞增殖和侵袭中的作用。采用逆转录定量PCR分析和蛋白质印迹法检测不同细胞系中AQP1的表达。构建了靶向AQP1的短发夹（sh）RNA并转染至MDA-MB-231乳腺癌细胞中。为了研究敲低AQP1的影响，通过细胞计数试剂盒8和Transwell实验评估乳腺癌细胞的增殖、迁移和侵袭能力。此外，测量小鼠体内肿瘤异种移植瘤的体积和重量以评估乳腺癌的生长能力。结果显示，与其他乳腺癌细胞系（MCF-7和SK-BR-3）以及正常乳腺上皮细胞系（MCF-10A）相比，MDA-MB-231细胞系中AQP1的水平更高。靶向AQP1的shRNA在mRNA和蛋白质水平有效下调AQP1表达，并显著抑制TNBC细胞的增殖、迁移和侵袭以及肿瘤生长。这些结果表明，AQP1可能是TNBC的一个潜在治疗靶点。
