Liang Hua, Luo Ruoyu, Chen Xiaoqi, Zhao Yuzi, Tan Aili
Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Oncol Rep. 2017 Oct;38(4):1977-1984. doi: 10.3892/or.2017.5916. Epub 2017 Aug 23.
MicroRNAs (miRNAs) are a cluster of short non-coding RNAs playing critical roles in human cancers. miR-187 was recently found to be a novel cancer-related microRNA. However, the expression and function of miR-187 in cervical cancer have not been investigated. In this study, we found that miR-187 level was decreased in cervical cancer tissues and cell lines. Patients with low level of miR-187 had significantly decreased rate of overall survival (OS) and progression-free survival (DFS). miR-187 overexpression inhibited proliferation and promoted apoptosis of cervical cancer cells, whereas miR-187 knockdown promoted proliferation and inhibited apoptosis of cervical cancer cells. Forced expression of miR-187 inhibited the subcutaneous growth of cervical cancer cells in nude mice. Furthermore, FGF9 was found to be the downstream target of miR-187 in cervical cancer cells. Importantly, targeting FGF9 was required for miR-187 exerting its tumor suppressive roles in cervical cancer cells.
微小RNA（miRNA）是一类短链非编码RNA，在人类癌症中发挥着关键作用。miR-187最近被发现是一种新型的癌症相关微小RNA。然而，miR-187在宫颈癌中的表达和功能尚未得到研究。在本研究中，我们发现miR-187水平在宫颈癌组织和细胞系中降低。miR-187水平低的患者总生存率（OS）和无进展生存率（DFS）显著降低。miR-187过表达抑制宫颈癌细胞增殖并促进其凋亡，而miR-187敲低则促进宫颈癌细胞增殖并抑制其凋亡。miR-187的强制表达抑制了宫颈癌细胞在裸鼠体内的皮下生长。此外，发现成纤维细胞生长因子9（FGF9）是宫颈癌细胞中miR-187的下游靶点。重要的是，miR-187在宫颈癌细胞中发挥其肿瘤抑制作用需要靶向FGF9。
