Bio‑center, Gyeonggi Institute of Science and Technology Promotion, Suwon, Gyeonggi 443‑270, Republic of Korea.
Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam 330‑714, Republic of Korea.
Mol Med Rep. 2017 Oct;16(4):5303-5311. doi: 10.3892/mmr.2017.7296. Epub 2017 Aug 21.
Tribulus terrestris L. (T. terrestris) has been used as a traditional medicine for the treatment of diuretic, lithontriptic, edema and urinary infections. Previous studies have indicated that it is effective in improving inflammation by regulating tumor necrosis factor‑α (TNF)‑α, interleukin (IL)‑6, IL‑10, nitric oxide (NO) and cyclooxygenase (COX)‑2. However, the effects and mechanism of action of T. terrestris on osteoarthritis (OA) remain unknown. Therefore, the present study aimed to evaluate the effects of the ethanolic extract of T. terrestris (ETT) in a monosodium iodoacetate (MIA)‑induced OA animal model. OA was induced in LEW/SSNHSD rats by intra‑articular injection of MIA. Morphometric changes and parameters of the tibial trabecular bone were determined using micro‑computed tomography. The molecular mechanisms of ETT in OA were investigated using reverse transcription‑polymerase chain reaction, western blotting and gelatin zymogram analysis. Treatment with ETT attenuated MIA‑induced OA, and this effect was mediated by the downregulation of NO synthase 2, COX‑2, TNF‑α and IL‑6. Furthermore, the ETT‑mediated attenuation of OA was also dependent on the expression of matrix metalloproteinases‑2 and ‑9. The results of the current study indicate that further evaluation of the mechanisms underlying the attenuation of MIA‑induced OA by ETT are required, and may support the development of ETT as a potential therapeutic agent for the treatment of inflammatory diseases such as OA.
蒺藜（T. terrestris）已被用作传统药物，用于治疗利尿、碎石、水肿和尿路感染。先前的研究表明，它通过调节肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6、IL-10、一氧化氮（NO）和环氧化酶（COX）-2，有效改善炎症。然而，蒺藜对骨关节炎（OA）的作用和作用机制尚不清楚。因此，本研究旨在评估蒺藜乙醇提取物（ETT）在碘乙酸单钠（MIA）诱导的 OA 动物模型中的作用。通过关节内注射 MIA 在 LEW/SSNHSD 大鼠中诱导 OA。使用微计算机断层扫描确定胫骨小梁骨的形态变化和参数。使用逆转录-聚合酶链反应、western blot 和明胶酶谱分析研究 ETT 在 OA 中的分子机制。ETT 减轻了 MIA 诱导的 OA，这种作用是通过下调一氧化氮合酶 2、COX-2、TNF-α和 IL-6 介导的。此外，ETT 介导的 OA 减轻也依赖于基质金属蛋白酶-2 和 -9 的表达。本研究的结果表明，需要进一步评估 ETT 减轻 MIA 诱导的 OA 的机制，这可能支持将 ETT 开发为治疗 OA 等炎症性疾病的潜在治疗剂。
