College of Basic Medical Sciences, Dalian Medical University, Dalian, China.
College of Veterinary Medicine, Jilin University, Changchun, China.
Adv Exp Med Biol. 2017;975 Pt 1:107-117. doi: 10.1007/978-94-024-1079-2_10.
Hexabromocyclododecane (HBCD) is a widely used brominated flame retardant. Its adverse effects on brain had been observed. Taurine, a sulfur amino acid, take part in many brain physiological functions and exhibits protective effects on a variety of detrimental situations. In this paper, we explored the protections of taurine on cytotoxicity induced by HBCD in PC12 cells. PC12 cells were pretreated with taurine (1 mM, 3 mM and 9 mM) for 30 min before 10 μM HBCD treatment for 24 h. Then, the cell survival was assayed by the lactate dehydrogenase (LDH) release and trypan blue dyeing method. The formation of reactive oxygen species (ROS) and a collapse of mitochondrial membrane potential (MMP) were evaluated with a fluorescence microplate reader using the non-fluorescent probe 2'7'-dichlorofluorescin diacetate (DCFH-DA) and the fluorescent cationic dyestuff Rhodamine 123 (Rh 123), respectively. Further, the activity of many antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and the content of glutathione (GSH) were tested by kits. Our results displayed that taurine significantly decreased the cell death induced by HBCD, prevented ROS production and disruption of mitochondrial membrane potential, and reversed the decline of SOD, CAT, GPx activity and GSH content induced by HBCD. These results suggested that taurine could alleviate cytotoxicity induced by HBCD in PC12 cells through inhibition of oxidative stress.
六溴环十二烷（HBCD）是一种广泛使用的溴系阻燃剂。已经观察到它对大脑有不良影响。牛磺酸是一种含硫氨基酸，参与许多大脑生理功能，并对各种有害情况表现出保护作用。在本文中，我们探讨了牛磺酸对 HBCD 诱导的 PC12 细胞毒性的保护作用。PC12 细胞在用 10 μM HBCD 处理 24 h 之前，用牛磺酸（1 mM、3 mM 和 9 mM）预处理 30 min。然后，通过乳酸脱氢酶（LDH）释放和台盼蓝染色法测定细胞存活率。使用非荧光探针 2'7'-二氯荧光素二乙酸酯（DCFH-DA）和荧光阳离子染料 Rhodamine 123（Rh 123），通过荧光微孔板读数器评估活性氧物种（ROS）的形成和线粒体膜电位（MMP）的崩溃。此外，通过试剂盒测试了许多抗氧化酶的活性，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽（GSH）的含量。我们的结果表明，牛磺酸可显著降低 HBCD 诱导的细胞死亡，防止 ROS 产生和线粒体膜电位破坏，并逆转 HBCD 诱导的 SOD、CAT、GPx 活性和 GSH 含量下降。这些结果表明，牛磺酸可以通过抑制氧化应激减轻 HBCD 诱导的 PC12 细胞毒性。
