Developmental Biology, Institute of Zoology, University of Cologne, Cologne, Germany.
Life Sciences Department, The Natural History Museum, London, United Kingdom.
Elife. 2017 Aug 29;6:e27590. doi: 10.7554/eLife.27590.
Organizers play important roles during the embryonic development of many animals. The most famous example is the Spemann organizer that sets up embryonic axes in amphibian embryos. In spiders, a group of BMP secreting mesenchymal cells (the cumulus) functions as an organizer of the dorsoventral axis. Similar to experiments performed with the Spemann organizer, transplantation of the cumulus is able to induce a secondary axis in spiders. Despite the importance of this structure, it is unknown which factors are needed to activate cumulus specific gene expression. To address this question, we performed a transcriptomic analysis of early embryonic development in the spider Through this work, we found that the transcription factor is needed for cumulus integrity, dorsoventral patterning and for the activation of and expression. Furthermore, ectopic expression of is sufficient to induce cell delamination and migration by inducing a mesoderm-like cell fate.
组织者在许多动物的胚胎发育过程中起着重要作用。最著名的例子是 Spemann 组织者，它在两栖动物胚胎中建立胚胎轴。在蜘蛛中，一群分泌 BMP 的间质细胞（卵丘）充当背腹轴的组织者。类似于用 Spemann 组织者进行的实验，卵丘的移植能够在蜘蛛中诱导次生轴。尽管这个结构很重要，但尚不清楚激活卵丘特异性基因表达需要哪些因素。为了解决这个问题，我们对蜘蛛的早期胚胎发育进行了转录组分析。通过这项工作，我们发现转录因子 对于卵丘的完整性、背腹模式的形成以及 和 的表达的激活是必要的。此外，异位表达 足以通过诱导类似中胚层的细胞命运来诱导细胞分层和迁移。
