Vaughan G M, Pruitt B A, Mason A D
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;87(1):71-4. doi: 10.1016/0742-8413(87)90183-6.
The in vitro response of melatonin synthesis was assessed by incubation of individual whole pineal glands for 4 hr without or with different concentrations of isoproterenol in the medium. Pineals were taken either at the end of the 14-hr light phase (day), or at 6 1/2 hr into the 10-hr dark phase (night) after a 30-min exposure of the animals to light just before sacrifice at night. The response was greater in pineals taken at night than in those taken at the end of the light phase in rats, but was absent at the end of the light phase in Syrian hamsters. Hamster pineals taken at night responded, though higher isoproterenol concentrations were required than in the rats. Unresponsiveness of hamster pineals during the day may explain previous failure of isoproterenol administration to stimulate pineal MEL content in this species.
通过在无或有不同浓度异丙肾上腺素的培养基中孵育单个完整松果体4小时，评估褪黑素合成的体外反应。松果体在14小时光照期结束时（白天）采集，或者在夜间处死动物前30分钟将动物暴露于光照后，在10小时黑暗期的6.5小时时采集。在大鼠中，夜间采集的松果体的反应比光照期结束时采集的松果体的反应更大，但在叙利亚仓鼠光照期结束时则无反应。夜间采集的仓鼠松果体有反应，不过所需的异丙肾上腺素浓度比大鼠中的更高。仓鼠松果体在白天无反应可能解释了先前在该物种中给予异丙肾上腺素未能刺激松果体褪黑素含量的原因。
