Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark, Kemitorvet Building 204, 2800 Kgs. Lyngby, Denmark.
Sci Rep. 2017 Aug 29;7(1):9683. doi: 10.1038/s41598-017-10092-9.
Bacterial antimicrobial resistance (AMR) in pigs is an important public health concern due to its possible transfer to humans. We aimed at quantifying the relationship between the lifetime exposure of antimicrobials and seven antimicrobial resistance genes in Danish slaughter pig farms. AMR gene levels were quantified by qPCR of total-community DNA in faecal samples obtained from 681 batches of slaughter pigs. The lifetime exposure to antimicrobials was estimated at batch level for the piglet, weaner, and finisher periods individually for the sampled batches. We showed that the effect of antimicrobial exposure on the levels of AMR genes was complex and unique for each individual gene. Several antimicrobial classes had both negative and positive correlations with the AMR genes. From 10-42% of the variation in AMR gene levels could be explained in the final regression models, indicating that antimicrobial exposure is not the only important determinant of the AMR gene levels.
猪的细菌抗菌药物耐药性（AMR）是一个重要的公共卫生关注点，因为它可能会转移到人类身上。我们旨在定量分析丹麦屠宰猪场中抗菌药物终生暴露与七种抗菌药物耐药基因之间的关系。通过对从 681 批屠宰猪的粪便样本中提取的总群落 DNA 进行 qPCR，定量了 AMR 基因水平。针对抽样批次，分别在仔猪、断奶仔猪和育肥猪阶段单独估算了抗菌药物在每个阶段的终生暴露量。我们表明，抗菌药物暴露对 AMR 基因水平的影响是复杂的，并且对每个基因都是独特的。一些抗菌药物类别与 AMR 基因既有负相关也有正相关。在最终的回归模型中，可以解释 AMR 基因水平变化的 10-42%，这表明抗菌药物暴露并不是 AMR 基因水平的唯一重要决定因素。
