Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.
Sci Rep. 2017 Aug 29;7(1):9699. doi: 10.1038/s41598-017-09945-0.
Soybean cyst nematode (SCN) is the most damaging pest of soybean worldwide. The molecular mechanism of SCN resistance remains largely unknown. We conducted a global RNA-seq comparison between a resistant genotype (S54) and a susceptible genotype (S67) of Glycine soja, the wild progenitor of soybean, to understand its regulatory network in SCN defense. The number of differentially expressed genes (DEGs) in S54 (2,290) was much larger than that in S67 (555). A number of defense-related genes/pathways were significantly induced only in S54, while photosynthesis and several metabolic pathways were affected in both genotypes with SCN infection. These defense-associated DEGs were involved in pathogen recognition, calcium/calmodulin-mediated defense signaling, jasmonic acid (JA)/ethylene (ET) and sialic acid (SA)-involved signaling, the MAPK signaling cascade, and WRKY-involved transcriptional regulation. Our results revealed a comprehensive regulatory network involved in SCN resistance and provided insights into the complex molecular mechanisms of SCN resistance in wild soybean.
大豆胞囊线虫（SCN）是全球范围内对大豆危害最大的害虫。SCN 抗性的分子机制在很大程度上仍不清楚。我们对大豆野生近缘种野生大豆的抗性基因型（S54）和感病基因型（S67）进行了全球 RNA-seq 比较，以了解其在 SCN 防御中的调控网络。S54 中的差异表达基因（DEGs）数量（2290 个）远大于 S67 中的数量（555 个）。许多与防御相关的基因/途径仅在 S54 中被显著诱导，而光合作用和几种代谢途径在感染 SCN 的两种基因型中都受到影响。这些与防御相关的 DEGs 参与了病原体识别、钙/钙调蛋白介导的防御信号、茉莉酸（JA）/乙烯（ET）和唾液酸（SA）参与的信号、MAPK 信号级联和 WRKY 参与的转录调控。我们的研究结果揭示了一个涉及 SCN 抗性的综合调控网络，并为深入了解野生大豆中 SCN 抗性的复杂分子机制提供了新的见解。
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