Yasmin Farida, Zhang Hengyou, Leamy Larry, Wang Baosheng, Winnike Jason, Reid Robert W, Brouwer Cory R, Song Bao-Hua
Department of Biological Sciences, The University of North Carolina at Charlotte, Charlotte, NC, United States.
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
Front Plant Sci. 2024 Feb 28;15:1240981. doi: 10.3389/fpls.2024.1240981. eCollection 2024.
Glyceollins, a family of phytoalexins elicited in legume species, play crucial roles in environmental stress response (e.g., defending against pathogens) and human health. However, little is known about the genetic basis of glyceollin elicitation. In the present study, we employed a metabolite-based genome-wide association (mGWA) approach to identify candidate genes involved in glyceollin elicitation in genetically diverse and understudied wild soybeans subjected to soybean cyst nematode. In total, eight SNPs on chromosomes 3, 9, 13, 15, and 20 showed significant associations with glyceollin elicitation. Six genes fell into two gene clusters that encode glycosyltransferases in the phenylpropanoid pathway and were physically close to one of the significant SNPs (ss715603454) on chromosome 9. Additionally, transcription factors (TFs) genes such as and were also found as promising candidate genes within close linkage to significant SNPs on chromosome 9. Notably, four significant SNPs on chromosome 9 show epistasis and a strong signal for selection. The findings describe the genetic foundation of glyceollin biosynthesis in wild soybeans; the identified genes are predicted to play a significant role in glyceollin elicitation regulation in wild soybeans. Additionally, how the epistatic interactions and selection influence glyceollin variation in natural populations deserves further investigation to elucidate the molecular mechanism of glyceollin biosynthesis.
大豆抗毒素是在豆科植物中产生的一类植物抗毒素，在环境应激反应（如抵御病原体）和人类健康方面发挥着关键作用。然而，关于大豆抗毒素诱导的遗传基础知之甚少。在本研究中，我们采用基于代谢物的全基因组关联（mGWA）方法，在遭受大豆胞囊线虫侵害的遗传多样且研究较少的野生大豆中，鉴定参与大豆抗毒素诱导的候选基因。总共，位于3号、9号、13号、15号和20号染色体上的8个单核苷酸多态性（SNP）与大豆抗毒素诱导表现出显著关联。6个基因落入两个基因簇，这些基因簇在苯丙烷途径中编码糖基转移酶，并且在物理位置上靠近9号染色体上的一个显著SNP（ss715603454）。此外，转录因子（TFs）基因如 和 也被发现是与9号染色体上显著SNP紧密连锁的有前景的候选基因。值得注意的是，9号染色体上的4个显著SNP表现出上位性和强烈的选择信号。这些发现描述了野生大豆中大豆抗毒素生物合成的遗传基础；所鉴定的基因预计在野生大豆的大豆抗毒素诱导调控中发挥重要作用。此外，上位性相互作用和选择如何影响自然种群中的大豆抗毒素变异，值得进一步研究以阐明大豆抗毒素生物合成的分子机制。
