Tokyo University of Science, Department of Applied Biological Science, Faculty of Science and Technology, 2641, Yamazaki, Noda, Chiba, 278-8510, Japan.
Nagoya University, Graduate School of Bioagricultural Sciences, School of Agricultural Sciences, Furo-cho, Chikusa, Nagoya, Aichi, 464-8601, Japan.
Sci Rep. 2017 Aug 29;7(1):9697. doi: 10.1038/s41598-017-10322-0.
The rice blast fungus Magnaporthe oryzae differentiates a specialized infection structure called an appressorium to invade rice cells. In this report, we show that CBP1, which encodes a chitin-deacetylase, is involved in the induction phase of appressorium differentiation. We demonstrate that the enzymatic activity of Cbp1 is critical for appressorium formation. M. oryzae has six CDA homologues in addition to Cbp1, but none of these are indispensable for appressorium formation. We observed chitosan localization at the fungal cell wall using OGA. This observation suggests that Cbp1-catalysed conversion of chitin into chitosan occurs at the cell wall of germ tubes during appressorium differentiation by M. oryzae. Taken together, our results provide evidence that the chitin deacetylase activity of Cbp1 is necessary for appressorium formation.
稻瘟病菌 Magnaporthe oryzae 分化出一种专门的侵染结构，称为附着胞，以侵染水稻细胞。在本报告中，我们表明，编码一种几丁质脱乙酰酶的 CBP1 参与附着胞分化的诱导阶段。我们证明了 Cbp1 的酶活性对附着胞形成至关重要。除了 Cbp1 之外，M. oryzae 还有六个 CDA 同源物，但它们都不是附着胞形成所必需的。我们使用 OGA 观察到壳聚糖在真菌细胞壁上的定位。这一观察结果表明，在稻瘟病菌附着胞分化过程中，Cbp1 催化的几丁质向壳聚糖的转化发生在芽管的细胞壁上。综上所述，我们的结果提供了证据表明，Cbp1 的几丁质脱乙酰酶活性对于附着胞的形成是必要的。
