Hamza Mohammed Falalu, Shaik Shakira, Moodley Roshila
School of Chemistry and Physics, University of Kwazulu Natal, Westville, Durban,4000, South Africa.
School of Life Sciences, University of KwaZulu- Natal, Westville, Durban, 4000, South Africa.
Afr J Tradit Complement Altern Med. 2016 Jul 3;13(4):74-80. doi: 10.21010/ajtcam.v13i4.11. eCollection 2016.
Existing populations of (Lauraceae) are rapidly declining as a consequence of their substitutive use for . The uncontrolled and excessive removal of the bark and roots of this species has led to the death of many of these plants and may eventually result in its depletion in the natural habitat.
The secondary metabolites from the leaves and fruits of were extracted using solvents of various polarities, isolated using column chromatography and identified using spectroscopic techniques. The free radical scavenging activity (antioxidant capacity) of selected phytocompounds at varied concentrations was determined by the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. A propagation study of the species was also conducted.
The compounds isolated from the plant were the novel compound, α-pyrone (5-hexyltetrahydro-2H-pyran-2-one) and known compounds quercetin-3--rhamnoside, β-sitosterol, copaene and nerolidol. The radical scavenging activity of the isolated compounds indicated moderate to good anti-oxidant activity. Treatment of explants with BAP: NAA at 1.0:0.01 mg L produced the highest percentage of shoots (94%) and longest shoot length (8.06 mm).
This study validates the ethno-medicinal use of the plant and supports the replacement of bark and roots by leaves and fruits for the management and conservation of this declining plant species. The benefits of consuming the fruits are two-fold as they can also contribute to the recommended dietary allowances of most essential elements for the majority of individuals.
由于被用作 的替代品，现有的 （樟科）种群数量正在迅速下降。对该物种树皮和根部的无节制过度砍伐导致许多此类植物死亡，并最终可能导致其在自然栖息地中枯竭。
使用各种极性的溶剂提取 叶片和果实中的次生代谢产物，通过柱色谱法进行分离，并使用光谱技术进行鉴定。通过2,2-二苯基-1-苦基肼（DPPH）测定法测定不同浓度下所选植物化合物的 自由基清除活性（抗氧化能力）。还对该物种进行了繁殖研究。
从该植物中分离出的化合物有新型化合物α-吡喃酮（5-己基四氢-2H-吡喃-2-酮）以及已知化合物槲皮素-3--鼠李糖苷、β-谷甾醇、古巴烯和橙花叔醇。分离出的化合物的自由基清除活性表明具有中度至良好的抗氧化活性。用1.0:0.01 mg L的BAP:NAA处理外植体产生了最高的芽百分比（94%）和最长的芽长度（8.06 mm）。
本研究验证了该植物的民族药用价值，并支持用叶片和果实替代树皮和根部，以管理和保护这种数量不断减少的植物物种。食用果实有双重益处，因为它们还可以为大多数人提供大多数必需元素的推荐膳食摄入量。
