Klopell Fernando Canani, Lemos Marivane, Sousa João Paulo Barreto, Comunello Eros, Maistro Edson Luis, Bastos Jairo Kennup, de Andrade Sérgio Faloni
Núcleo de Ciência e Tecnologia, Area de Ciências Biológicas e da Saúde, Universidade do Oeste de Santa Catarina, Campus de Videira, Rua Paese, 198, Bairro Universitário, Videira, SC, CEP 89560-900, Brazil.
Z Naturforsch C J Biosci. 2007 Jul-Aug;62(7-8):537-42. doi: 10.1515/znc-2007-7-812.
In this study, the antiulcerogenic effect of essential oil from Baccharis dracunculifolia was evaluated using the model of acute gastric lesions induced by ethanol. The ulcerative lesion index (ULI) was significantly reduced by oral administration of the essential oil of B. dracunculifolia at doses of 50, 250 and 500 mg/kg which reduced the lesions by 42.79, 45.70 and 61.61%, respectively. The analysis of the chemical composition of the essential oil from B. dracunculifolia by GC showed that this was composed mainly of mono- and sesquiterpenes and the majority compound was nerolidol. Therefore, antiulcerogenic activity of nerolidol (50, 250 and 500 mg/kg) was investigated using ethanol-, indomethacin- and stress-induced ulcer models in rat. In the stress-induced ulcer model, a significant reduction of the ULI in animals treated with nerolidol (50, 250 and 500 mg/kg) and cimetidine (100 mg/kg) was observed, compared to the control group (p < 0.05). The percentage of inhibition of ulcer was 41.22, 51.31, 56.57 and 53.50% in groups treated with 50, 250, 500 mg/kg of nerolidol and 100 mg/kg of cimetidine (positive control), respectively. Regarding ethanol- and indomethacin-induced ulcer models, it was observed that the treatment with nerolidol (250 and 500 mg/ kg) significantly reduced the ULI in comparison with the control group (p < 0.05). The dose of 50 mg/kg reduced the parameters analyzed but this was not statistically significant. In the ethanol-induced model percentage of inhibition of ulcer was 34.20, 52.63, 87.63 and 50.87% in groups treated with 50, 250, 500 mg/kg of nerolidol and 30 mg/kg of omeprazol (positive control), respectively. In indomethacin-ulcer the percentage of inhibition of ulcer was 34.69, 40.80, 51.02 and 46.93% in groups treated with 50, 250, 500 mg/kg of nerolidol and 100 mg/ kg of cimetidine (positive control), respectively. The results of this study show that nerolidol displays antiulcer activity, as it significantly inhibited the formation of ulcers induced in different animal models. However, further pharmacological and toxicological investigations, to delineate the mechanism(s) of action and the toxic effects, are required to allow the use of nerolidol for the treatment of gastric ulcer.
在本研究中，使用乙醇诱导的急性胃损伤模型评估了巴西龙蒿精油的抗溃疡作用。口服50、250和500mg/kg剂量的巴西龙蒿精油可显著降低溃疡病变指数（ULI），分别使损伤减少42.79%、45.70%和61.61%。通过气相色谱法对巴西龙蒿精油的化学成分分析表明，其主要由单萜和倍半萜组成，其中含量最多的化合物是橙花叔醇。因此，使用乙醇、吲哚美辛和应激诱导的大鼠溃疡模型研究了橙花叔醇（50、250和500mg/kg）的抗溃疡活性。在应激诱导的溃疡模型中，与对照组相比，观察到用橙花叔醇（50、250和500mg/kg）和西咪替丁（100mg/kg）处理的动物的ULI显著降低（p<0.05）。用50、250、500mg/kg的橙花叔醇和100mg/kg的西咪替丁（阳性对照）处理的组中，溃疡抑制率分别为41.22%、51.31%、56.57%和53.50%。关于乙醇和吲哚美辛诱导的溃疡模型，观察到与对照组相比，用橙花叔醇（250和500mg/kg）处理可显著降低ULI（p<0.05）。50mg/kg的剂量降低了所分析的参数，但无统计学意义。在乙醇诱导的模型中，用50、250、500mg/kg的橙花叔醇和30mg/kg的奥美拉唑（阳性对照）处理的组中，溃疡抑制率分别为34.20%、52.63%、87.63%和50.87%。在吲哚美辛诱导的溃疡中，用50、250、500mg/kg的橙花叔醇和100mg/kg的西咪替丁（阳性对照）处理的组中，溃疡抑制率分别为34.69%、40.80%、51.02%和46.93%。本研究结果表明，橙花叔醇具有抗溃疡活性，因为它能显著抑制不同动物模型中溃疡的形成。然而，需要进一步的药理和毒理学研究来阐明其作用机制和毒性作用，以便将橙花叔醇用于治疗胃溃疡。
