Khan Inam Ullah, Hussain Firasat, Habib Neeli, Xiao Min, Ahmed Iftikhar, Amin Arshia, Zhi Xiao-Yang, Li Wen-Jun
Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2848-2852. doi: 10.1099/ijsem.0.002032.
A novel actinobacterial strain, designated NCCP-696T, was isolated from the Thal desert in Punjab, Pakistan, and characterized by using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NCCP-696T belongs to the genus Nocardioides and showed the highest level of sequence similarity with respect to Nocardioides panacisoliGsoil 346T (98.2 %) and less than 96.4 % to the strains of other species of the genus Nocardioides. Cells of strain NCCP-696T were Gram-positive, aerobic, non-motile rods and formed cream-coloured colonies. The strain was positive for oxidase and catalase. Growth occurred at 20-42 °C (optimum 30-37 °C) at pH 5.5-9.0 (optimum pH 7.0) and with 0-4 % NaCl (optimum 0-2 %, w/v). Strain NCCP-696T contained Iso-C16 : 0, C18 : 1ω9c, C17 : 1ω8c and C17 : 0 as the predominant fatty acids and was found to have LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan. The polar lipid profile consisted of phosphatidylinositol, phosphatidylglycerol and one unknown phospholipid. The major menaquinone was MK-8(H4) (98.7 %) while a minor amount (1.3 %) of MK-9(H2) was also detected. The DNA G+C content of the genomic DNA was 71.6 mol%. The DNA-DNA hybridization value of the isolate against the closely related type strain Nocardioides panacisoliGsoil 346T was 56.3±1.4. On the basis of the phylogenetic inference, chemotaxonomic characteristics and phenotypic data, strain NCCP-696T should be classified as a novel species, for which the name Nocardioides thalensis sp. nov. is proposed. The type strain is NCCP-696T (=DSM 103833T=CCTCC AB 2016296T).
从巴基斯坦旁遮普省的塔尔沙漠中分离出了一株新型放线菌，命名为NCCP-696T，并采用多相分类学方法对其进行了表征。基于16S rRNA基因序列的系统发育分析表明，菌株NCCP-696T属于诺卡氏菌属，与诺卡氏菌属人参土壤杆菌Gsoil 346T的序列相似性最高（98.2%），与诺卡氏菌属其他物种的菌株序列相似性低于96.4%。菌株NCCP-696T的细胞为革兰氏阳性、需氧、不运动的杆菌，形成奶油色菌落。该菌株氧化酶和过氧化氢酶呈阳性。在20-42°C（最适温度30-37°C）、pH值5.5-9.0（最适pH值7.0）和0-4% NaCl（最适0-2%，w/v）条件下生长。菌株NCCP-696T以异-C16:0、C18:1ω9c、C17:1ω8c和C17:0作为主要脂肪酸，细胞壁肽聚糖中含有LL-2,6-二氨基庚二酸。极性脂质谱由磷脂酰肌醇、磷脂酰甘油和一种未知磷脂组成。主要的甲基萘醌是MK-8(H4)（98.7%），同时还检测到少量（1.3%）的MK-9(H2)。基因组DNA的G+C含量为71.6 mol%。该分离株与密切相关的模式菌株诺卡氏菌属人参土壤杆菌Gsoil 346T的DNA-DNA杂交值为56.3±1.4。基于系统发育推断、化学分类学特征和表型数据，菌株NCCP-696T应被分类为一个新物种，为此提出了新种名为诺卡氏菌属塔尔沙漠种（Nocardioides thalensis sp. nov.）。模式菌株为NCCP-696T（=DSM 103833T=CCTCC AB 2016296T）。
