Nocardioides silvaticus sp. nov., isolated from forest soil.

A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34 was isolated from forest soil. According to 16S rRNA gene sequence analysis, strain S-34 was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696 (97.3 %) and Nocardioides panacisoliGsoil 346 (97.0 %), Nocardioides litorisoli X-2 (96.5 %) and Nocardioides immobilis FLL521 (96.4 %). Phylogenetic trees showed that strain S-34 fell within the cluster containing strain S-34 and N. immobilis FLL521. The levels of DNA-DNA relatedness between strain S-34 and N. thalensis CCTCC AB 2016296 and between strain S-34 and N. panacisoli KCTC 19470 were 50.6 and 58.8 %, respectively. The genome orthoANI value between strain S-34 and N. immobilis CCTCC AB 2017083 was 82.4 %. Strain S-34 had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids. The genome length of strain S-34 was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%. Strain S-34 could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences. Therefore, strain S-34 represents a novel species of the genus Nocardioides, for which the name Nocardioidessilvaticus sp. nov. is proposed. The type strain is S-34 (=KCTC 49137=CCTCC AB 2018079).