State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat‑Sen University, Guangzhou, 510275, People's Republic of China.
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 Nov 25;118(1):32. doi: 10.1007/s10482-024-02046-9.
Two novel strains, SYSU D00514 and SYSU D00778, were isolated from desert soil in the Gurbantunggut Desert, Xinjiang Uygur Autonomous Regions, PR China. SYSU D00514 and SYSU D00778 were aerobic, Gram-stain-positive, rod-shaped, catalase-positive and oxidase-negative. SYSU D00514 grew at temperatures ranging from 4 to 37 °C (optimum, 28-30 °C), at pH 6.0-8.0 (optimum, 7.0), and tolerated NaCl concentrations from 0 to 7.5% (optimum, 0.5% w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains SYSU D00514 and SYSU D00778 were closely related to the members of the genus Nocardioides. Two isolates were most closely related to Nocardioides alpinus Cr7-14 (98.05% and 98.19%). The G + C content of the genomic DNA of strains SYSU D00514 and SYSU D00778 were both 72.8%. Strain SYSU D00514 and the type strains of the genus Nocardioides in the analysis had average nucleotide identity values of 76.8-85.8% as well as digital DNA-DNA hybridization values between 19.5% and 28.0%. The predominant cellular fatty acids of strain SYSU D00514 were C ω8c, C ω9c and iso-C. For strain SYSU D00514, the major polar lipids encompassed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, an unidentified aminophospholipid and two unidentified aminolipids. The main respiratory quinone of strain SYSU D00514 was MK-8 (H4). Based on the results of polyphasic taxonomic experiments, we propose that strains SYSU D00514 and SYSU D00778 represent a novel species of the genus Nocardioides, with the name Nocardioides xinjiangensis. The type strain is SYSU D00514 (= CGMCC 1.18622 = MCCC 1K05001 = KCTC 49488).
两株新菌株，SYSU D00514 和 SYSU D00778，从中国新疆维吾尔自治区古尔班通古特沙漠的土壤中分离得到。SYSU D00514 和 SYSU D00778 是需氧、革兰氏阳性、杆状、过氧化氢酶阳性和氧化酶阴性。SYSU D00514 在 4 至 37°C（最佳温度为 28-30°C）、pH 值 6.0-8.0（最佳 pH 值为 7.0）和耐受 0 至 7.5%（最佳为 0.5%w/v）的 NaCl 浓度下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 SYSU D00514 和 SYSU D00778 与诺卡氏菌属的成员密切相关。这两个分离株与高山诺卡氏菌 Cr7-14（98.05%和 98.19%）最为密切相关。菌株 SYSU D00514 和 SYSU D00778 的基因组 DNA 的 G+C 含量均为 72.8%。在分析中，SYSU D00514 菌株与诺卡氏菌属的模式菌株的平均核苷酸同一性值为 76.8-85.8%，数字 DNA-DNA 杂交值为 19.5%至 28.0%。菌株 SYSU D00514 的主要细胞脂肪酸为 C ω8c、C ω9c 和 iso-C。对于菌株 SYSU D00514，主要的极性脂包括双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、磷脂酰肌醇、一种未鉴定的氨基磷脂和两种未鉴定的氨基脂。菌株 SYSU D00514 的主要呼吸醌为 MK-8（H4）。基于多相分类学实验的结果，我们提出菌株 SYSU D00514 和 SYSU D00778 代表诺卡氏菌属的一个新种，命名为新疆诺卡氏菌。模式菌株为 SYSU D00514（=CGMCC 1.18622=MCCC 1K05001=KCTC 49488）。
