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土壤蓝细菌分离、纯化及培养的现代方法

Modern Methods for Isolation, Purification, and Cultivation of Soil Cyanobacteria.

作者信息

Temraleeva A D, Dronova S A, Moskalenko S V, Didovich S V

出版信息

Mikrobiologiia. 2016 Jul;85(4):369-380.

Abstract

Up-to-date methods for isolation of cyanobacteria from soil samples, removal of accompanying microflora, obtaining axenic strains, and -conditions and media for subsequnt cultivation are reviewed. Char acterization of soil as a specific habitat for cyanobacteria is provided. Comparative analysis of pH and ele- mental composition of the liquid phase of most soil types with the media for cultivating cyanobacteria is car- ried out. The functional role of the major components required for the cultivation of cyanobacteria is de- scribed. The problems associated with isolation, purification, and cultivation of soil cyanobacteria, as well as the relevant solutions, are discussed.

摘要

综述了从土壤样品中分离蓝藻、去除伴生微生物菌群、获得无菌菌株以及后续培养的条件和培养基的最新方法。介绍了土壤作为蓝藻特定栖息地的特征。对大多数土壤类型液相的pH值和元素组成与蓝藻培养基进行了比较分析。描述了培养蓝藻所需主要成分的功能作用。讨论了与土壤蓝藻分离、纯化和培养相关的问题以及相应的解决方法。

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