使微藻培养物再次无菌 - 利用荧光激活细胞分选的快速简单工作流程。
Make microalgal cultures axenic again - a fast and simple workflow utilizing fluorescence-activated cell sorting.
机构信息
Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria.
Research Division Biochemical Engineering, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien, Gumpendorfer Strasse 1a, 1060 Vienna, Austria.
出版信息
J Microbiol Methods. 2021 Jul;186:106256. doi: 10.1016/j.mimet.2021.106256. Epub 2021 May 31.
Since the removal of contaminations in microalgal cultures is extremely laborious and time-consuming, we developed a rapid workflow to obtain axenicity by a combination of fluorescence-activated cell sorting (FACS) and plate spreading. During method development, several cyanobacteria and green algae strains were successfully made axenic. At the end, method transferability to another FACS device was demonstrated. Our workflow offers great time-savings with less hands-on laboratory work compared to conventional isolation techniques.
由于微藻培养物中的污染物去除非常费力且耗时，因此我们开发了一种快速工作流程，通过荧光激活细胞分选 (FACS) 和平板铺展的组合来获得无菌性。在方法开发过程中，成功地使几种蓝藻和绿藻菌株无菌。最后，证明了该方法可转移到另一台 FACS 设备上。与传统的分离技术相比，我们的工作流程大大节省了时间，减少了实验室的手工操作。
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